Characterization and Linkage Mapping of an ENU-Induced Mutant Mouse with Defective Spermatogenesis

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  • ASANO Yuka
    Graduate School of Natural Science and Technology, Okayama University
  • AKIYAMA Kouyou
    Graduate School of Natural Science and Technology, Okayama University
  • TSUJI Takehito
    Graduate School of Natural Science and Technology, Okayama University
  • TAKAHASHI Sakino
    Tokyo University of Agriculture and Technology
  • NOGUCHI Junko
    Reproductive Biology Research Unit, National Institute of Agrobiological Sciences
  • KUNIEDA Tetsuo
    Graduate School of Natural Science and Technology, Okayama University

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抄録

repro23 is an autosomal recessive mutation of the mouse generated by the N-ethyl-N-nitrosourea (ENU)-induced mutagenesis program at The Jackson Laboratory. The repro23/repro23 homozygous mouse shows male-specific infertility caused by defective spermatogenesis. In the present study, we investigated the testicular pathology of the affected mouse and performed linkage analysis to determine the chromosomal localization of the repro23 locus. Histological examination of the affected testis showed that the seminiferous epithelium of the repro23/repro23 mice contained spermatogonia and early stage spermatocytes, but no spermatids or spermatozoa. Immunohistochemical staining for Hsc70t, a spermatid specific protein, confirmed the absence of elongating spermatids. These findings indicated interruption of the spermatogenesis during meiosis in the repro23/repro23 mouse. By linkage analysis using 137 affected mice of F2 progeny obtained from crosses between repro23/repro23 female and JF1/Ms (+/+) male mice, the repro23 locus was mapped to 2.2-Mb region of mouse chromosome 7. Although this region contains several potential candidate genes for the repro23 mutation, no gene already identified as a cause of defective speramatogenesis was in this region. Therefore, the gene responsible for the repro23 mutation is suggested to be a novel gene which plays an essential role in mammalian spermatogenesis.<br>

収録刊行物

  • Experimental Animals

    Experimental Animals 58 (5), 525-532, 2009

    公益社団法人 日本実験動物学会

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