Safe And Efficient Collection of Cytokine-Mobilized Peripheral Blood Cells From Cynomolgus Monkeys (Macaca fascicularis) with Human Newborn-Equivalent Body Weights
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- AGEYAMA Naohide
- Tsukuba Primate Research Center, National Institute of Biomedical Innovation Corporation for Production and Research of Laboratory Primates
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- HANAZONO Yutaka
- Center for Molecular Medicine, Jichi Medical School
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- SHIBATA Hiroaki
- Tsukuba Primate Research Center, National Institute of Biomedical Innovation Center for Molecular Medicine, Jichi Medical School
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- ONO Fumiko
- Corporation for Production and Research of Laboratory Primates
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- OGAWA Hiromi
- Corporation for Production and Research of Laboratory Primates
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- NAGASHIMA Takeyuki
- DNAVEC Corporation
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- UEDA Yasuji
- DNAVEC Corporation
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- YOSHIKAWA Yasuhiro
- Department of Biomedical Science, Graduate School of Agriculture and Life Science, University of Tokyo
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- HASEGAWA Mamoru
- DNAVEC Corporation
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- OZAWA Keiya
- Center for Molecular Medicine, Jichi Medical School
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- TERAO Keiji
- Tsukuba Primate Research Center, National Institute of Biomedical Innovation
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Abstract
Hematopoietic stem cells in bone marrow can be mobilized into peripheral blood by cytokine administration. Cytokine-mobilized peripheral blood stem cells are of great use in clinical applications. We previously established a modified procedure for the collection of cytokine-mobilized peripheral blood cells from rhesus monkeys (Macaca mulata) using a commercially available apparatus originally developed for human subjects. In this study, we examined the efficacy and safety of this method with even smaller macaques, cynomolgus monkeys (Macaca fascicularis), which are equivalent to human newborns in body weight (mean = 3.3 kg). Using the manufacturer's unmodified protocol (n=6), one monkey died of cardiac failure and three developed severe anemia. In contrast, using our modified procedure (n=6), no such complication was observed in any animal. In addition, the harvested nuclear cell, mononuclear cell and CD34+ cell counts were significantly higher with the modified method. The modified method should allow safe and efficient collection of cytokine-mobilized peripheral blood cells from non-human primates as small as human newborns in a non-invasive manner.<br>
Journal
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- Experimental Animals
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Experimental Animals 54 (5), 421-428, 2005
Japanese Association for Laboratory Animal Science
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Details 詳細情報について
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- CRID
- 1390001205046457472
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- NII Article ID
- 10016785510
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- NII Book ID
- AA11032321
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- ISSN
- 18817122
- 00075124
- 13411357
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- NDL BIB ID
- 7681186
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed