Studies on Clostridium perfringens

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Other Title
  • Clostridium perfringensの研究
  • Clostridium perfringensの研究-2-S型抗原とR型抗原
  • Clostridium perfringens ノ ケンキュウ 2 Sガタ コウゲン ト Rガタ コウゲン
  • 2. S-form and R-form antigens
  • 第2報S型抗原とR型抗原

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Description

The stabilities of S and R-form antigens of 4 strains of Clostridium perfringens prepared with 4 kinds of media (Basic (BA), Trypto-soy (TA), Heart infusion (HIA) and CW agar plate) were examined by agglutination tests against 41 of antisera.<BR>1. Colonies of the rugged structure were found always on HIA and CW. The antigens prepared with these colonies were agglutinated by 41 of antisera in higher and wider area than those of BA and TA.<BR>2. The budding of R-form was observed almost as a rhizoid segment from the periphery of a Scolony. It occurred in high level from the first plating of the stock culture. A little increase of incidence of R-form was observed on BA and TA, much increase on HIA and CW by incubating for 48 hr, but not 72 hr.<BR>3. When 8 R-antigens and 4 antigens of their parent S-strains were compared by the agglutination tests agalinst 41 of antisera, 5 types of R-antigens were found, namely, the perfectly same, partial difference, complete difference etc. However, if more R-strains were examined, R-antigens of much more different character would have appeared.<BR>4. By absorption test the R-strain differed completely from the parent strain in the antigenic structure. The agglutination tests of R-antigens against 14 of human normal sera showed parallel relation to the result against above-mentioned 41 antisera. Particularly, one R-antigen of the widest positive reaction-area showed 1: 80-320 of titer by all normal sera. These agglutinations were presumed to be non-specific.<BR>5. By these all results, to prepare the antigen of Clostridium perfringens author recommend to use BA as the medium, to purify S-form by the several subcultures and to incubate less than for 20 hr.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 48 (1), 1-7, 1974

    The Japanese Association for Infectious Diseases

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