Anti-Vertoxin-Neutralizing Antibody in Intravenous Gammaglobulin Preparations

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  • ADACHI Eriko
    Department of Infectious Diseases Research, National Children's Medical Research Center
  • YOSHINO Ken-ichi
    Department of Infectious Diseases Research, National Children's Medical Research Center
  • KIMURA Tsuyoshi
    Teijin Institute for Bio-Medical Research
  • MATSUMOTO Yoh-ichi
    Teijin Institute for Bio-Medical Research
  • TAKEDA Tae
    Department of Infectious Diseases Research, National Children's Medical Research Center

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Other Title
  • 静注用γ-グロブリン製剤中のベロ毒素中和抗体の検討
  • ジョウチュウヨウガンマ グロブリン セイザイチュウ ノ ベロ ドクソ チュウ

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Abstract

Intravenously administered immune globulin therapy has been reported to be an effective treatment for serious patients with verotoxin-producing Escherichia coli infections while an efficacy for VT2 are under discussion. We therefore examined in vitro commercially available immune globulin preparations for the presence of anti-VT neutralizing antibodies in expectation of protecting a patient from serious complications as HUS. We examined 47 lots of-globulin prepared by 5 pharmaceutical companies in Japan. Of them 29 lots were prepared from γ imported blood and 18 lots were from Japanese donors' blood. They were prepared in each company following their own manufactures' process.<BR>Neutralizing activity for VT1 and VT2 were determined by means of cytotoxicity using ACHN (renal adenocarcinoma, human) cells. All lots of γ-globulin preparations from imported blood completely neutralized 125pg/ml VT1 at the concentration of 12.5mg/ml, and no significant difference was found in the manufactures' process. On the other hand γ-globulin preparations from Japanese donors' blood neutralized VT1 five times less than the former. None of all preparations neutralized VT2.<BR>Gammaglobulin preparations were produced from pooled human plasma. To know the difference of neutralizing activities in the source, we examined randomly selected 239 human plasma samples of which 51 were from Japanese donors' blood and 188 were from imported blood. Prevalences of neutralizing activity of VT1 in imported plasma and domestic plasma were10.6% and 2.0%, respectively. The prevalence of neutralizing antibody n these plasma samples reflects the different neutralizing activity of VT1 in γ-globulin preparations prepared from imported blood and Japanese donors' blood.<BR>From these results, by selecting the lot of γ-globulin preparations or the material of plasma with highly neutralizing activity, intravenously administered immune globulin therapy may be effective for VT1. The lack of VT2-neutralizing activity in any γ-globulin preparation promotes us to develop humanized anti-VT2-monoclonal antibody for the prevention of HUS in high risk children.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 72 (8), 808-812, 1998

    The Japanese Association for Infectious Diseases

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