Typing of Group B Streptococci by Counterimmunoelectophoresis

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  • KOBAYASHI Sadao
    Department of Bioorganic Chemistry, College of Environmental Health, Azabu University
  • MATSUOKA Yuko
    Department of Bioorganic Chemistry, College of Environmental Health, Azabu University
  • KORI Yoshio
    Chiba Municipal Hospital
  • MURAI Teiko
    Department of Public Health, School of Medicine, Toho University

Bibliographic Information

Other Title
  • CounterimmunoelectrophoresisによるB群溶連菌の型別
  • CounterimmunoelectrophoresisによるB群溶連菌の型別--沈降反応法およびスライド凝集反応法との比較
  • Counterimmunoelectrophoresis ニ ヨル Bグン ヨ
  • Comparison with Precipitin Method and Slide Agglutination Method
  • 沈降反応法およびスライド凝集反応法との比較

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Description

The counterimmunoelectrophoresis method (CIE) was studied applicability to typing of group B streptococci.Types obtained by CIE were compared with those by the capillary precipitin method (CPP) and the slide agglutination method (SAG).<BR>Preparation of antigens for typing of group B streptococci (GBS) has been performed by hot HC1 extraction at 50°C or 100°C, saline extraction and autoclave extraction method.The antigens prepared by 50C HC1 extraction and autoclave extraction showed identical reactivity and specificity, whereas the other antigens showed poor reactivity and many cross-reaction. Autoclave extraction is recommend for typing of GBS by CIE, because preparation of the antigens is rapid and relatively easy.<BR>The antigens prepared by autoclave extraction from 16 strains of GBS isolated from patients were typed by CIE and the results were compared with types obtained by CPP and SAG.Polysaccharide antigen types obtained by CIE were in agreement with the results obtained by CPP and SAG.In 15 out of 16 strains “c” protein antigen type agreed with the results obtained by CPP and in the remaining one “c”protein antigen was not detected by CPP and SAG, but it was typed by CIE.This finding suggests a higher sensitivity of CIE in comparison with CPP and SAG.By the SAG method, 9 strains, in which “c” protein antigen was detected by CPP and CIE, showed various heat-labile protein antigen types, Q, S, or W.In the remaining 5 strains, in which “c” protein antigen was not detected by CPP and CIE, possessed no heat-labile protein antigens.<BR>Detection of type antigen from three specimens of serum and concentrated urine obtained from neonates suffering from meningitis caused by GBS performed by CIE.Type III antigen was detected in the two concentrated urine samples and it was the same serological type as the strain isolated from the patient.In the serum obtained from GBS type Ic patient, no type antigen was detected.<BR>The CIE method as applied to group B streptococci typing is specific, highly sensitive, rapid and relatively easy.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 59 (9), 852-859, 1985

    The Japanese Association for Infectious Diseases

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