レプトスピラ症の血清診断への蛍光抗体法間接法の応用に関する研究

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タイトル別名
  • Studies of Application of Indirect Fluorescent Antibody Technique for Serodiagnosis of Leptospirosis
  • レプトスピラ症の血清診断への蛍光抗体法間接法の応用に関する研究-2-患者と野犬の血清を対象とした実用経験
  • レプトスピラショウ ノ ケッセイ シンダン エ ノ ケイコウ コウタイホウ カ
  • 2. Experimental Studies on Sera from Patients and Stray Dogs
  • 2. 患者と野犬の血清を対象とした実用経験

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抄録

Application of indirect fluorescent anti-leptospiral antibody technique (FLAi) to serodiagnosis of leptospirosis in humans and dogs was studied. Six serotypes of leptospira found in Japan were used as the antigen, they were fixed onto slide glass using Kirkpatrik's solution (Ethanol: ether: formalin=6: 3: 1). The procedure of FLAi was arranged after the FTA-ABS method for serological diagnosis of syphilis. Dried Fluorescein-Conjugated Goat Antiserum to Human IgM (μ-chain specific) Laboratory Reagent (Hyland) and Fluorescein-Labelled Anti-Human γ-Globulin (mainly IgG) Rabbit Serum (Eiken Chemical) were used. Anti-dog γ-Globulin Fluorescent Antibody was prepared by the author. For screening of positive antisera, a mixture of equal amounts of the 6 serotypes was used, the test sera were examined at 5- and 20-fold dilutions.<BR>The antibody titer of the sera from patients of Weil's disease was higher forFLAi than for microscopic agglutination (MA). The sera from 17 undoubted patients at acute stage from whose bloods serotype pyrogenes was isolated, were unexceptionally negative in MA, whereas 9/17 cases to copenhageni antigen and 12/17 cases to the mixed antigen were positive in FLAi (IgM). The serum fromthe convalescent stage showed serotype specificity in MA, whereas considerable cross reactivities inFLAi. There was not so much difference between FLAi (IgM) and FLAi (IgG) titers both in the acute and convalescent sera, nor any consistent tendency between MA and FLAi (IgG) titers in the convalescent sera. From these results, it was considered to be more desirable to use the labelled serum containing both anti-IgM and anti-IgG antibodies for serodiagnosis of human leptospirosis.<BR>In the sera from 4 patients at more than 5 years after the infection, FLAi demonstrated the antibody at the same grade with MA. Out of 5 MA negative sera, one was positive when they were examined at 5-fold dilution, but the others negative. By screening of 295 serum samples from the patients with liver complaints using the mixed antigen of 6 serotypes, 18 were positive at 5-folddilution of the samples and 5 at 20-fold dilution. After absorption with Reiter's antigen, all except 5 turned negative.<BR>FLAi was not always genus-specific in dog, some giving high titer against copenhageni and others against canicola. In the sera of 3 dogs with doubtless infection (1 disease and 2 carrying), MA detected higher titer than FLAi in all cases. It was concluded that the mixed antigen was more adequate than the single serotype antigen for screening purpose in both dogs and human patients.

収録刊行物

  • 感染症学雑誌

    感染症学雑誌 54 (8), 374-382, 1980

    一般社団法人 日本感染症学会

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