Comparison of short chain fatty acids detected in pus of experimentally induced subcutaneous abscesses in mice by non-sporing anaerobes, culture medium and human clinical specimens by gas-liquid chromatography

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  • NARIKAWA Shinichi
    Department of Laboratory Medicine, St. Marianna University School of Medicine
  • NAKASHIO Satoshi
    Department of Laboratory Medicine, St. Marianna University School of Medicine
  • NAKAMURA Masao
    Department of Laboratory Medicine, St. Marianna University School of Medicine
  • HARASAWA Isao
    Bacteriology Section, Clinical Laboratory, St. Marianna Medical School Hospital

Bibliographic Information

Other Title
  • 無芽胞嫌気性菌の実験的マウス単独感染皮下膿瘍と培養培地および患者検体中の低級脂肪酸のガスクロマトグラフィー (GLC) による比較について
  • ム ガホウ ケンキセイキン ノ ジッケンテキ マウス タンドク カンセン ヒカ

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Abstract

Five percent mucin suspensions each containing Bacteroides fragilis, Fusobacterium varium, Peptococcus asaccharolyticus, and Peptostreptococcus anaerobius isolated from anaerobic infections were injected once subcutaneously into the dd strain of conventional mice weighing 14 to 16 g. Bacteroides asaccharolyticus Rm-1 and Veillonella alcalescens ATCC 17745 were injected in the same way. Injections consisted of 0.2 ml. Viable cell number of bacteria per ml of mucin suspensions were approximately 2 x108 colony forming units (CFU) of Bacteroides fragilis, 4×107 CFU of F. varium, 3×107 CFU of P. asaccharolyticus, 1×107 CFU of P. anaerobius, 2×108 CFU of B. asaccharolyticus, and 4×107 CFU of V. alcalescens. Injections of pure culture of each organism except B. asaccharolyticus produced subcutaneous abscess of back. Injection of pure culture of B. asaccharolyticus produced moist gangrene. The lesions were visible 8 to 10 days after injection of each organism. The injected organisms were recovered from each of the lesion and no contaminating aerobe or other anaerobes were found within these lesions. Pus obtained from subcutaneous abscess in mice were analyzed by the gasliquid chromatograph (GLC) equipped with flame ionization detectors for the presence of short chain fatty acids. Chromatographic patterns of volatile fatty acids had strongly resemblance to culture results, however non-volatile fatty acids had not. GLC analysis of clinical specimens of pus recovered mixed anaerobes revealed that combined chromatographic patterns of volatile fatty acids detected in pus of subcutaneous abscesses inmice were obtained.<BR>It is considered that GLC analysis of iso-butyric acid, butyric acid, iso-valeric acid, valeric acid, isocaproic acid and caproic acid, which were not detectable in clinical specimens of pus from aerobic infections, provides reliable means for the presumptive diagnosis of infections caused by anaerobes and for identification of the pathogen.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 56 (6), 457-465, 1982

    The Japanese Association for Infectious Diseases

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