Evaluation of the Culturette Brand Ten-Minute Group A Strep ID test Regard to its Specificity and Sensitivity

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Other Title
  • Culturette Brand Ten-Minute Group A Strep IDの基礎的検討
  • Culturette Brand Ten Minute Group A S

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It has been well known that C-polysaccharide antigen of group A streptococci is extracted by nitrous acid and the agglutinating reaction is observed between this antigen and anti-C-polysaccharide antibody coated latex particles.Recently, a latex agglutination kit (Culturette Brand Ten-Minute Group A Strep ID) for the identification of group A streptococci directly from throat swabs was developed as a useful and highly rapid technique for the application of clinical use.<BR>The purpose of this investigation was to evaluate this kit in regard to its specificity and sensitivity.<BR>The results obtained were as follows:<BR>1) The agglutinating reactions appeared by using 10-1 to 10-6 diluted fluids of the original group A streptococcal cell suspension (1.4×108 cells/ml) and latex particles coating anti-group A Cpolysaccharide antibody, but the positive reaction was not observed by employing 10-7 diluted fluid of the cells.<BR>2) Group A streptococcal cell suspension only reacted with latex particles coating anti-group A St. C-polysaccharide antibody, but group B St., group C St., group D St., group G St., St.pneumoniae, St. sanguis, St.mitior, St.mutans, S.aureus and S.epidermidis did not react with the latex particles.<BR>3) Identification of group A St.by Strep ID test was not affected by the existence of various bacterial strains.<BR>4) The latex particles coating anti-group A St.C-polysaccharide antibody reacted with group A St. C-polysaccharide (50ng/ml), but it did not react with C-polysaccharide of St.pneumoniae and Protein-A.<BR>5) The reaction of strep ID test was inhibited by the addition of N-acetyl-glucosamine.<BR>Therefore, the Strep ID test is considered to be a sensitive and specific tool to identify group A St.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 59 (12), 1204-1209, 1985

    The Japanese Association for Infectious Diseases

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