Transferability of <I>VanA</I> Gene from Vancomycin-Resistant <I>Enterococcus faecalis</I> in the Digestive Tract of Specific Pathogen-Free Mice

DOI Web Site PubMed 17 References
  • IKEDA Tatsuo
    Department of Microbiology and Immunology, Teikyo University School of Medicine
  • WATANABE Takeshi
    Department of Microbiology and Immunology, Teikyo University School of Medicine
  • MATSUMOTO Kaoru
    Department of Microbiology and Immunology, Teikyo University School of Medicine
  • MURAYAMA Somay Y.
    Kitasato Institute for Life Science & Graduate School of Infection Control Sciences Laboratory of Infectious Agents Surveillance
  • KOSHIO Osamu
    Department of Microbiology and Immunology, Teikyo University School of Medicine
  • TANSHO Shigeru
    Department of Microbiology and Immunology, Teikyo University School of Medicine
  • ONO Yasuo
    Department of Microbiology and Immunology, Teikyo University School of Medicine

Bibliographic Information

Other Title
  • マウス腸管内におけるvancomycin-resistant <I>Enterococcus faecalis vanA</I>遺伝子の伝達性
  • マウス腸管内におけるvancomycin-resistant Enterococcus faecalis vanA遺伝子の伝達性
  • マウス チョウ カンナイ ニ オケル vancomycin resistant Enterococcus faecalis vanA イデンシ ノ デンタツセイ
  • Transferability of VanA Gene from Vancomycin-Resistant Enterococcus faecalis in the Digestive Tract of Specific Pathogen-Free Mice

Search this article

Description

We evaluated the transferability of vanA gene from vancomycin-resistant Enterococcus faecalis (VREF) to vancomycin-sensitive E. faecalis (VSEF) in vitro and in vivo. In vitro conjugal transfer experiment by filter mating, the vanA gene of VREF was transferable at the high frequency to VSEF and a mutant strain which cured vanA gene of VREF. In vivo studies in the digestive tract of specific pathogen-free mice pretreated with oral antibiotics, transconjugants were also detected from the feces of a mouse at the lower frequency. However, the colonization of transconjugants was transient. The vanA gene in the donor and the transconjugant strain was confirmed by using a polymerase chain reaction method.<BR>These results suggest that VSEF colonizing in the human digestive tract might be developed to VREF by transferring of the vanA gene.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 78 (11), 952-958, 2004

    The Japanese Association for Infectious Diseases

References(17)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top