Effect of the Mixed-Oxidant Solution on Infectivity of <I>Cryptosporidium parvum</I> Oocysts in a Neonatal Mouse Model

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  • 混合酸化剤溶液の<I>Cryptosporidium parvum</I>オーシスト感染性に対する不活化効果
  • 混合酸化剤溶液のCryptosporidium parvumオーシスト感染性に対する不活化効果
  • コンゴウ サンカザイ ヨウエキ ノ Cryptosporidium parvum オーシスト カンセンセイ ニ タイスル フカツカ コウカ
  • Effect of the Mixed-Oxidant Solution on Infectivity of Cryptosporidium parvum Oocysts in a Neonatal Mouse Model

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Abstract

Cryptosporidium parvum oocysts were exposed to the mixed-oxidant solution, which was electrochemically generated by Miox Water Disinfection Unit, and sodium hypochlorite in phosphate buffered saline (PBS, pH 7.2) or biologically treated wastewater at 25°C by using concentrations of residual chlorine of up to 5 mg/l and contact times of up to 8 h. The effect of two disinfectants on infectivity of the oocysts in a neonatal murine model was comparatively evaluated by determining the total number of oocysts recovered from the intestine. Exposure to the mixed-oxidant solution at 2 and 5 mg/l (residual chlorine) yielded a significant inactivation of infectivity in the dose-and exposure timedependent manner, while exposure to 5 mg/l (residual chlorine) of sodium hypochlorite for contact times of up to 4 h produced no measurable inactivation of infectivity. Morphological examination also revealed a picture of degenerating oocysts after exposure to 5 mg/l (residual chlorine) of the mixedoxidant solution, but not with sodium hypochlorite. When the oocysts were exposed to either biologically treated wastewater-or PBS-diluted the mixed-oxidant solution at 5 mg/l (residual chlorine) for4 h, the disinfectants produced a significant inactivation of infectious oocysts. The decrease number of the oocysts was 0.8 log10 in the former and 2.1 log10 in the latter. These results demonstrate that the mixed-oxidant solution may be a useful disinfectant against Cryptosporidium oocysts, but appropriate applications need to be validated.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 77 (2), 75-82, 2003

    The Japanese Association for Infectious Diseases

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