Development of an Enzyme-Linked Immunosorbent Assay (ELISA) for Detection of IgM and IgG Antibodies to Lipopolysaccharide of <I>Escherichia coli</I> O157

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  • ELISA法による腸管出血性大腸菌O157由来LPS IgMおよびIgG抗体測定の基礎および臨床的検討
  • ELISAホウ ニ ヨル チョウカン シュッケツセイ ダイチョウキン O157 ユライ LPS IgM オヨビ IgG コウタイ ソクテイ ノ キソ オヨビ リンショウテキ ケントウ
  • Development of an Enzyme-Linked Immunosorbent Assay (ELISA) for Detection of IgM and IgG Antibodies to Lipopolysaccharide of Escherichia coli O157

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Abstract

We developed the enzyme-linked immunosorbent assay (ELISA) for detection of serum IgM and IgG antibodies to lipopolysaccharide (LPS) of Escherichia coli O157: H7 (VT1+, VT2+) isolated from a clinical sample. We tested the patient's sample with hemolytic uremic syndrome (HUS) and hemorrhagic colitis (HC). And we evaluated the value of sample OD to cut off control OD as the Index. The CVs obtained in precision studies were below 10% for intra-and inter-assay, respectively. The cut off index value of infants and children (0to5years) was 0.60 for IgM and 0.95 for IgG. Moreover we investigated the specificity to E. coli O157from other E. coli serotypes in this method. From these results, O27 serotype was crossreacted in this assay, but other serotypes were not reacted. In the patients with HUS and HC, some cases were already positive for IgM at 3days, but for IgG8days were needed for it to become positive. We concluded that this ELISA for IgM and IgG for E. coli O157 LPS have a useful performance, and might be the useful tool for rapid diagnosis for E. coli O157 infection.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 73 (6), 593-599, 1999

    The Japanese Association for Infectious Diseases

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