Survey on Epidemic of Mycoplasma pneumoniae pneumonia by Isolation Method

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  • OKAZAKI Norio
    Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratories
  • AKEMA Riichirou
    Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratories
  • TAKIZAWA Kinjirou
    Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratories

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Other Title
  • 分離検査によるMycoplasma pneumoniae肺炎流行の追跡
  • ブンリ ケンサ ニ ヨル Mycoplasma pneumoniae ハイエン

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Abstract

Survey on pneumonia caused by Mycoplasma pneumoniae was performed in Kanagawa prefecture from 1983 to 1985. The disease was diagnosed by isolation of M. pneumoniae from throat swabs of patients with primary atypical pneumonia (PAP). In addition, several kinds of media were examined on the utility for M. pneumoniae isolation.<BR>A total of 173 strains of M. pneunomiae (35%) were isolated from 493 PAP patients for 3 years. The number of isolates and isolation rate in each year were as follows; 23 (24%) in 1983, 115 (51%) in 1984 and 35 (20%) in 1985. Thus, the highest isolation rate was recognized in 1984. The pattern of monthly isolation in 1984 showed that epidemic of M. pneumoniae pneumonia occurred in the period from June to November, with an peak in July.<BR>M. pneumoniae pneumonia began to appear among school children chiefly aged 7 to 9 years in the latter half of pre-epidemic year. Although the occurrence of the disease in those children increased in June of epidemic year, a total occurrence in children aged 4 to 12 year increased in the period of summer vacation from July to August. These results suggested that M. pneumoniae infection was introduced into families by the school children above and that infection in families occurred frequently during summer season with the epidemic peak.<BR>Pneumonia caused by M. pneumoniae were found frequently in children aged 4 to 12 years, whereas the occurrence of the disease was a little observed in children less than 3 years old. The incidence of the disease in PAP increased with age of the children.<BR>As for isolation medium of M. pneumoniae, diphasic medium containing 15% of heated (56°C, 30min) horse serum was slightly more effective than ordinary one containing 20% of unheated serum. Diphasic medium containing 10% of gammaglobulin free calf serum and egg yolk medium were useful for M. pneumoniae isolation. As compared with the unheated horse serum medium above, these two media shortened incubation days for the isolation, whereas the isolation rates by them were lower. Therefore, it was considered that combination use of these three media was more effective for M. pneumoniae isolation.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 61 (5), 547-554, 1987

    The Japanese Association for Infectious Diseases

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