Isolation of Shiga Toxin-producing <I>Escherichia coli</I> O157: H7 from Processed Salmon Roe Associated with the Outbreaks in Japan, 1998, and a Molecular Typing of the Isolates by Pulsed-field Gel Electrophoresis
-
- ASAI Yoshio
- Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory
-
- MURASE Toshiyuki
- Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory
-
- OSAWA Ro
- Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory
-
- OKITSU Tadayuki
- Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory
-
- SUZUKI Rieko
- Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory
-
- SATA Shin
- Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory
-
- YAMAI Shiro
- Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory
-
- TERAJIMA Jun
- Deparent of Bacteriology, National Institute of Infectious Diseases
-
- IZUMIYA Hidemasa
- Deparent of Bacteriology, National Institute of Infectious Diseases
-
- TAMURA Kazumichi
- Deparent of Bacteriology, National Institute of Infectious Diseases
-
- WATANABE Haruo
- Deparent of Bacteriology, National Institute of Infectious Diseases
Bibliographic Information
- Other Title
-
- イクラからの志賀毒素産生性大腸菌O157: H7の分離と分離株のパルスフィールドゲル電気泳動パターン
- イクラ カラ ノ シガ ドクソ サンセイセイ ダイチョウキン O157 H7 ノ ブンリ ト ブンリカブ ノ パルスフィールド ゲル デンキ エイドウ パターン
- Isolation of Shiga Toxin-producing Escherichia coli O157: H7 from Processed Salmon Roe Associated with the Outbreaks in Japan, 1998, and a Molecular Typing of the Isolates by Pulsed-field Gel Electrophoresis
Search this article
Abstract
Shiga toxin-producing Escherichia coil (STEC) O157 were isolated from processed salmon roe which had been a suspected food item in sporadic infections which occurred in Japan in 1998. A total of 45 samples of the processed salmon roe were pre-enriched in trypticase soy broth (TSB) at 36°C for 6 h and novobiocin-supplemented modified EC broth (mEC-NB) at 42°C for 18 h. After the preenrichments, the cultures were examined for possible occurrence of STEC O157, using an immunomagnetic separation (IMS) method. From the examination, a total of 84 strains of STEC O157: H7 that were positive for both stx 1 and stx 2 genes were isolated. By applying the most-probablenumber technique, it was estimated that the number of STEC O157 was in the range of 0.73-1.5 per 10 g of the processed salmon roe.<BR>Subsequent analysis of the isolates by a pulsed-field gel electrophoresis (PFGE) revealed a pattern commonly seen in 82 isolates and another pattern in two isolates. Clinical isolates from 7 patients also showed an identical pattern to those of the 82 isolates and one isolate from a patient showed the other pattern identical to those of the two isolates. The isolates were found to belong to the phage type 14.
Journal
-
- Kansenshogaku Zasshi
-
Kansenshogaku Zasshi 73 (1), 20-24, 1999
The Japanese Association for Infectious Diseases
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390001205051003776
-
- NII Article ID
- 130004330694
- 10008713974
-
- NII Book ID
- AN00047715
-
- ISSN
- 1884569X
- 03875911
-
- NDL BIB ID
- 4649217
-
- PubMed
- 10077898
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed