Usefulness of Arbitrarily Primed Polymerase Chain Reaction (AP-PCR) Method for DNA Fingerprinting Analysis of <I>Mycobacterium tuberculosis</I> (MTB); Comparison between DNA Restriction Fragment-Length Polymorphism (RFLP) Method and AP-PCR Method

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  • MUKAIGAWA Jun
    Department of Microbiology, Tokyo Metropolitan Institute of Public Health
  • SHIMOJIMA Yukako
    Department of Microbiology, Tokyo Metropolitan Institute of Public Health
  • MURATA Iwao
    Department of Microbiology, Tokyo Metropolitan Institute of Public Health
  • ENDOU Miyoko
    Department of Microbiology, Tokyo Metropolitan Institute of Public Health
  • YANAGAWA Yositoki
    Department of Microbiology, Tokyo Metropolitan Institute of Public Health
  • MOROZUMI Satosi
    Department of Microbiology, Tokyo Metropolitan Institute of Public Health

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Other Title
  • 結核集団感染の分子疫学的解析におけるArbitrarily Primed PCR (AP-PCR) 法の有用性
  • ケッカク シュウダン カンセン ノ ブンシエキガクテキ カイセキ ニ オケル Arbitrarily Primed PCR AP PCR ホウ ノ ユウヨウセイ
  • Usefulness of Arbitrarily Primed Polymerase Chain Reaction (AP-PCR) Method for DNA Fingerprinting Analysis of Mycobacterium tuberculosis (MTB); Comparison between DNA Restriction Fragment-Length Polymorphism (RFLP) Method and AP-PCR Method

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Description

We have been analyzing cases suspected as outbreak of Mycobacterium tuberculosis in Tokyo using RFLP method. This time we analyzed 27 strains of MTB from 5 cases in two hospitals, a family, member of social activity and stuff of a corporation using both RFLP and AP-PCR methods. At 4 cases, over 80% of strains were same pattern in each cases with RFLP and AP-PCR and were identified as a patient to patients transmission of MTB. At one case, in a hospital, each strains were completely different patterns at both methods, which showed it was not a outbreak case. Results of RFLP and AP-PCR were completely same, which indicates AP-PCR is also useful and rapid method for epidemiological analysis of MTB infection as well as RFLP.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 77 (12), 1040-1048, 2003

    The Japanese Association for Infectious Diseases

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