ANALYSIS OF ANTIGENS RECOGNIZED BY ANTI-γ-SEMINOPROTEIN ANTIBODY AND ANTI-PROSTATE-SPECIFIC ANTIGEN ANTIBODY BY MEANS OF OUCHTERLONY METHOD AND WESTERN BLOTTING METHOD

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  • Ouchterlony 法および Western blotting 法による抗γ-seminoprotein 抗体および抗 prostate-specific antigen 抗体の認識抗原の比較検討
  • ANALYSIS OF ANTIGENS RECOGNIZED BY ANTI-^|^gamma;-SEMINOPROTEIN ANTIBODY AND ANTI-PROSTATE-SPECIFIC ANTIGEN ANTIBODY BY MEANS OF OUCHTERLONY METHOD AND WESTERN BLOTTING METHOD

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Antigens recognized by anti-γ-seminoprotein (γ-Sm) antibody and anti-prostate-specific antigen (PA) antibody were analyzed immunologically by Ouchterlony method and Western blotting method. The double immunodiffusion test demonstrated that anti-γ-Sm antibody and anti-PA antibody reacted against an identical antigen in seminal fluid or prostate homogenate. In the immunoblotting examination, anti-γ-Sm antibody and anti-PA antibody formed a single and identical band, which gave a molecular weight of 33, 000, in seminal fluid and prostate homogenate. This study demonstrated that anti-γ-Sm antibody and anti-PA antibody recognized a single and identical antigen in seminal fluid and prostate homogenate and suggested that an identical material could be measured in sera of patients with prostate cancer by immunoassay systems using anti-γ-Sm antibody and anti-PA antibody.

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