Reverse Genetics System for Measles Virus: Establishment and Applications for Analysis of Virus Replication and Pathogenesis

DOI Web Site 1 Citations 39 References
  • TAKEDA Makoto
    Department of Virology, Faculty of Medicine, Kyushu University

Bibliographic Information

Other Title
  • 麻疹ウイルス遺伝子操作系の確立と，複製および病原性発現の分子基盤の解析
  • マシン ウイルス イデンシ ソウサケイ ノ カクリツ ト フクセイ オヨビ ビョウゲンセイ ハツゲン ノ ブンシ キバン ノ カイセキ

Search this article

Abstract

In 1990 Kobune et al. succeeded in isolating pathogenic wild-type strains of measles virus (MV) using a marmoset B-lymphoblastoid cell line B95a. Their data indicated that MV strains that have been used in laboratories are attenuated strains through the adaptation to grow in a variety of cultured cells. We have established a very efficient reverse genetics system that allows us to engineer the genome of a wild-type MV strain at will by site-directed mutagenesis or recombination. Using the system it was shown that (1) the H protein determines tropism of MV, (2) the M protein regulates mode of MV spread, (3) the C protein inhibits host innate immune responses, and (4) the long untranslated regions in the M and F genes function to moderate cytopathogenicity by MV. These data advanced our understanding of molecular bases for MV pathogenicity and mechanisms of MV adaptation to grow in cultured cells.

Journal

  • Uirusu

    Uirusu 57 (1), 83-90, 2007

    The Japanese Society for Virology

Citations (1)*help

See more

References(39)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top