風疹ウイルス感染症における血清学的研究―とくにクラス別免疫グロブリン抗体の測定法とその消長について―

DOI Web Site PubMed

書誌事項

タイトル別名
  • STUDIES ON SPECIFIC IMMUNOGLOBULIN RESPONSES IN SERUM AFTER SPONTANEOUS INFECTION WITH RUBELLA VIRUS
  • フウシン ウイルス カンセンショウ ニ オケル ケッセイガクテキ ケンキュウ
  • I. DETECTION OF RUBELLA IgM AND IgA HEMAGGLUTINATION INHIBITION ANTIBODIES
  • I. 黄色ブドウ球菌 Cowan I株処理によるIgMおよびIgA風疹赤血球凝集抑制抗体の測定法

この論文をさがす

抄録

Rubella-specific IgM antibody in the serum of patients with natural rubella was investigated by the absorption of IgG with Staphylococcus aureus Cowan 1 (S. aureus) reported by Ankerst et al. and by sucrose density gradient centrifugation. The method of detection of rubella-specific IgA antibody was studied by a combination of the two methods mentioned above.<br>The present investigation confirmed that serum IgG was specifically removed by the absorption with S. aureus, but that an excess amount of S. aureus removed IgM and IgA nonspecifically from serum. An adequate amount of S. aureus that could remove most IgG from serum without affecting the IgM and IgA content was 0.1g (wet volume) in 0.3ml of serum diluted 1:8 with kaolin treatment or 0.2g of S. aureus in 0.3ml of serum diluted 1:8 without kaolin treatment.<br>Rubella HI antibodies were not demonstrated after the absorption with S. aureus in the serum of subjects having suffered from infection more than 5 years before, but in the serum of subject having recent infection, i.e., the serum obtained during a period from 3 to 115 days after the onset of rash. These results were coincident with the presence of rubella-specific IgM HI antibodies demonstrated by sucrose density gradient centrifugation. Rubella-specific IgA HI antibody in serum could be estimated by the fractionation of sucrose density gradient centrifugation after the absorption with S. aureus.

収録刊行物

  • ウイルス

    ウイルス 28 (2), 87-94, 1978

    日本ウイルス学会

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ