A reverse genetics system for flaviviruses

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  • SUZUKI Ryosuke
    Department of Virology II, National Institute of Infectious Diseases
  • KONISHI Eiji
    BIKEN Endowed Department of Dengue Vaccine Development, Faculty of Tropical Medicine, Mahidol University

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  • フラビウイルスのリバースジェネティクス
  • フラビウイルス ノ リバースジェネティクス

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Description

Flaviviruses such as Japanese encephalitis virus, West Nile virus, yellow fever virus, dengue virus, and tick-borne encephalitis virus belong to a family Flaviviridae. These viruses are transmitted to vertebrates by infected mosquitoes or ticks, producing diseases, which have a serious impact on global public health. Reverse genetics is a powerful tool for studying the viruses. Although infectious full-length clones have been obtained for multiple flaviviruses, their early-stage development had the difficulty because of the instability problem of the viral cDNA in E. coli. Several strategies have been developed to circumvent the problem of infectious clone instability. The current knowledge accumulated on reverse genetics system of flaviviruses and its application are summarized in this review.

Journal

  • Uirusu

    Uirusu 63 (1), 13-22, 2013

    The Japanese Society for Virology

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