書誌事項
- タイトル別名
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- Detection of DNA Damaging Agents Using Altered DNA Sequence of the Escherichia Coli RecA Gene.
- カイヘン recA イデンシ オ リヨウ シタ DNA ソンショウセイ ブッシ
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抄録
A bacterial test method was developed for detecting various DNA damaging agents by using the promoter-operator region of Escherichia coli SOS gene fused to the Vibrio harveyi luxAB genes. A E. coli strain containing the recA promoter-operator region (recApo)-luxAB fusion gene emitted light in a dose-dependent manner for the well-known DNA damaging agents mitomycin C (MMC), methyl methanesulfonate (MMS), and 4-nitroquinoline-1-oxide (4NQO). To make the response more sensitive, 4 base pairs of the recA operator sequence were changed, resulting in new promoter-operator sequence (po34) which had the consensus sequence for the LexA repressor binding. The E. coli strain containing po34-luxAB fusion gene showed 2-20 times higher sensitivity than the strain containing recApo-luxAB gene in the response to MMC, MMS, and 4NQO, because of its low background luminescence. As a comparison of the results of this method and the umu test for various compounds, the former had high sensitivity in 8 of the 14 samples compared with the latter.
収録刊行物
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- 水環境学会誌
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水環境学会誌 21 (6), 347-352, 1998
公益社団法人 日本水環境学会
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詳細情報 詳細情報について
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- CRID
- 1390001205080395008
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- NII論文ID
- 10007469132
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- NII書誌ID
- AN10372439
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- COI
- 1:CAS:528:DyaK1cXltVWltLo%3D
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- ISSN
- 18813690
- 09168958
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- NDL書誌ID
- 4494325
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
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- 抄録ライセンスフラグ
- 使用不可