Estimation of Results from Simplified Medaka Vitellogenin Assay for Xenoestrogens
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- HATANO Yasuhiko
- EnBioTec Laboratories Co., Ltd.
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- CHIKAE Miyuki
- Japan Advanced Institute of Science and Technology, Hokuriku
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- NISHI Kazuto
- EnBioTec Laboratories Co., Ltd.
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- TATARAZAKO Norihisa
- National Institute for Environmental Studies
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- MIZUKAMI Haruki
- EnBioTec Laboratories Co., Ltd.
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- YAMASHITA Michiaki
- National Research Institute of Fisheries Science
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- TAMIYA Eiichi
- Japan Advanced Institute of Science and Technology, Hokuriku
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- SAKAKIBARA Ryuzo
- Kyushu Women's University
Bibliographic Information
- Other Title
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- 簡易メダカ・ビテロジェニンアッセイによる外因性エストロジェンの影響評価研究
- 技術報告 簡易メダカ・ビテロジェニンアッセイによる外因性エストロジェンの影響評価研究
- ギジュツ ホウコク カンイ メダカ ビテロジェニンアッセイ ニ ヨル ガイインセイ エストロジェン ノ エイキョウ ヒョウカ ケンキュウ
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Abstract
A medaka (Oryzias latipes) vitellogenin bioassay is a powerful and useful tool for the screening of estrogenic compounds. In order to establish a rapid and simple method for the screening of estrogenic contaminants in an aquatic environment, we studied the effects of several test conditions on the induction of plasma vitellogenin in male medaka. During the holding period, treatments with animal drugs or NaCl for the prevention of an infective disease did not affect the induction of vitellogenin in male medaka. However, plasma vitellogenin in some male medaka was induced when they were kept with female or in water from a fish-breeding tank. During the testing period, vitellogenin induction in male medaka was not affected by the photoperiod cycle and temperature. However, this induction was affected by the frequency of water changing. In the present paper, we propose a rapid and simple method for the screening of estrogenic contaminants in an aquatic environment using a medaka vitellogenin assay. We also present the exposure test results of 4-nonylphenol and bisphenol A obtained using our simple method.
Journal
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- Journal of Japan Society on Water Environment
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Journal of Japan Society on Water Environment 26 (11), 779-785, 2003
Japan Society on Water Environment
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Details 詳細情報について
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- CRID
- 1390001205081232384
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- NII Article ID
- 10012445547
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- NII Book ID
- AN10372439
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- COI
- 1:CAS:528:DC%2BD2cXptFak
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- ISSN
- 18813690
- 09168958
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- NDL BIB ID
- 6761119
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed