An Evaluation Method of Bacterial Flora in Contaminated Soils -For the Purpose of a Countermeasure against H<SUB>2</SUB>S Gas Production-

  • TANIGUCHI Hatsumi
    Department of Microbiology, School of Medicine, University of Occupational and Environmental Health
  • FUKUDA Kazumasa
    Department of Microbiology, School of Medicine, University of Occupational and Environmental Health
  • YAN Wan
    Department of Microbiology, School of Medicine, University of Occupational and Environmental Health
  • HINOUE Mitsuo
    Department of Microbiology, School of Medicine, University of Occupational and Environmental Health
  • YAMAUCHI Kazuki
    Department of Microbiology, School of Medicine, University of Occupational and Environmental Health
  • ICHIHARA Tsuyoshi
    Department of Microbiology, School of Medicine, University of Occupational and Environmental Health
  • MIZUNO Kouhei
    Department of Materials Science and Chemical Engineering, Kitakyushu National College of Technology
  • ISHIMATSU Sumiyo
    Department of Environmental Management Ⅰ, School of Health Sciences, University of Occupational and Environmental Health
  • SERA Nobuyuki
    Division of Virology, Department of Health Science, Fukuoka Institute of Health and Environmental Sciences
  • HAMASAKI Mitsuhiro
    Division of Pathology and Bacteriology, Department of Health Science, Fukuoka Institute of Health and Environmental Sciences
  • TAKAHASHI Kouji
    Department of Environmental Science, Division of Waste Management, Fukuoka Institute of Health and Environmental Sciences
  • HORIKAWA Kazumi
    Division of Pathology and Bacteriology, Department of Health Science, Fukuoka Institute of Health and Environmental Sciences

Bibliographic Information

Other Title
  • 土壌細菌叢評価法の構築 -廃棄物処分場の硫化水素ガス発生対策のために-
  • ドジョウ サイキンソウ ヒョウカホウ ノ コウチク ハイキブツ ショブンジョウ ノ リュウカ スイソ ガス ハッセイ タイサク ノ タメニ

Search this article

Abstract

Gas productions in illegal dumping sites and waste landfills have caused serious problems. The gas production was induced by bacterial flora inhabited soils. In order to construct a culture independent evaluation system of the soil bacteria, bacterial communities were analyzed quantitatively and qualitatively, about 16 soil samples at 4 sites, both using culture and culture-independent methods. The real time PCR method was developed for counting total bacterial number. Sequencing analysis of 16S rDNA amplified by a direct PCR method revealed that non-spore forming sulfate reducing bacteria and sulfur-oxidizing bacteria were detected at a similar frequency at an illegal dumping site near mountains. On the other hand, spore-forming sulfate reducing bacteria and Clostridium sp. were mainly detected in deep samples at reclaimed landfills from the sea, whereas sulfur-oxidizing bacteria was hardly detected. The result regarding sulfate-reducing bacteria was confirmed also by an anaerobic culture method. Culture-independent molecular analyses of soil bacteria would give us useful information for prediction of gas production and for the evaluation of soil equilibrium.

Journal

  • Journal of UOEH

    Journal of UOEH 26 (3), 349-367, 2004

    The University of Occupational and Environmental Health, Japan

Citations (5)*help

See more

Details 詳細情報について

Report a problem

Back to top