An Evaluation Method of Bacterial Flora in Contaminated Soils －For the Purpose of a Countermeasure against H<SUB>2</SUB>S Gas Production－

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  • TANIGUCHI Hatsumi
    Department of Microbiology, School of Medicine, University of Occupational and Environmental Health
  • FUKUDA Kazumasa
    Department of Microbiology, School of Medicine, University of Occupational and Environmental Health
  • YAN Wan
    Department of Microbiology, School of Medicine, University of Occupational and Environmental Health
  • HINOUE Mitsuo
    Department of Microbiology, School of Medicine, University of Occupational and Environmental Health
  • YAMAUCHI Kazuki
    Department of Microbiology, School of Medicine, University of Occupational and Environmental Health
  • ICHIHARA Tsuyoshi
    Department of Microbiology, School of Medicine, University of Occupational and Environmental Health
  • MIZUNO Kouhei
    Department of Materials Science and Chemical Engineering, Kitakyushu National College of Technology
  • ISHIMATSU Sumiyo
    Department of Environmental Management Ⅰ, School of Health Sciences, University of Occupational and Environmental Health
  • SERA Nobuyuki
    Division of Virology, Department of Health Science, Fukuoka Institute of Health and Environmental Sciences
  • HAMASAKI Mitsuhiro
    Division of Pathology and Bacteriology, Department of Health Science, Fukuoka Institute of Health and Environmental Sciences
  • TAKAHASHI Kouji
    Department of Environmental Science, Division of Waste Management, Fukuoka Institute of Health and Environmental Sciences
  • HORIKAWA Kazumi
    Division of Pathology and Bacteriology, Department of Health Science, Fukuoka Institute of Health and Environmental Sciences

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Other Title
  • 土壌細菌叢評価法の構築 －廃棄物処分場の硫化水素ガス発生対策のために－
  • ドジョウ サイキンソウ ヒョウカホウ ノ コウチク ハイキブツ ショブンジョウ ノ リュウカ スイソ ガス ハッセイ タイサク ノ タメニ

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Abstract

Gas productions in illegal dumping sites and waste landfills have caused serious problems. The gas production was induced by bacterial flora inhabited soils. In order to construct a culture independent evaluation system of the soil bacteria, bacterial communities were analyzed quantitatively and qualitatively, about 16 soil samples at 4 sites, both using culture and culture-independent methods. The real time PCR method was developed for counting total bacterial number. Sequencing analysis of 16S rDNA amplified by a direct PCR method revealed that non-spore forming sulfate reducing bacteria and sulfur-oxidizing bacteria were detected at a similar frequency at an illegal dumping site near mountains. On the other hand, spore-forming sulfate reducing bacteria and Clostridium sp. were mainly detected in deep samples at reclaimed landfills from the sea, whereas sulfur-oxidizing bacteria was hardly detected. The result regarding sulfate-reducing bacteria was confirmed also by an anaerobic culture method. Culture-independent molecular analyses of soil bacteria would give us useful information for prediction of gas production and for the evaluation of soil equilibrium.

Journal

  • Journal of UOEH

    Journal of UOEH 26 (3), 349-367, 2004

    The University of Occupational and Environmental Health, Japan

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