Establishment of a Mouse Model to Assess Brain Neurotransmitter Level and Learning Performance Simultaneously Following Toxic Chemical Exposure : Using <i>in vivo</i> Microdialysis and Schedule-Controlled Operant Behavior

WIN-SHWE Tin-Tin, HOJO Rieko, MITSUSHIMA Dai, NAKAJIMA Daisuke, YAMAMOTO Shoji, FUJIMAKI Hidekazu

doi:10.7888/juoeh.31.1

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Other Title

インビボマイクロダイアリシスとオペラント行動を用いて有害化学物質曝露後の神経伝達物質と学習行動を同時に評価するための動物モデルの確立
Establishment of a mouse model to assess brain neurotransmitter level and learning performance simultaneously following toxic chemical exposure: using in vivo microdialysis and schedule-controlled operant behavior

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Abstract

Most of the volatile organic compounds used in industrial and household products are reported to be toxic. While the effects of toxic chemicals on pulmonary and other systemic functions have been studied substantially, little is known about their effects on higher brain functions, particularly on the events in learning performance. From the particular perspective of environmental health, it is necessary to examine the toxic effects of environmental chemicals on higher brain functions. The aim of the present study is to establish a mouse model for simultaneous assessment of the effect of toluene (150mg/kg, i.p.) on extracellular glutamate level in the hippocampus, using in vivo microdialysis and learning performance with a procedure of schedule-controlled operant behavior (SCOB). Different patterns of hippocampal glutamate level were observed in control and toluene-treated mice during learning performance. During learning performance in an operant chamber, control mice showed a saturated and persistent increased glutamate level with good learning performance, while toluene-treated mice showed a decreased glutamate level with poor learning performance. This is the first time to establish a new method to assess the effect of environmental chemical exposure on the brain neurotransmitter level during learning performance in an animal model.

Journal

Journal of UOEH

Journal of UOEH 31 (1), 1-11, 2009

The University of Occupational and Environmental Health, Japan

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