Studies on a Simultaneous Analytical Method of Urinary Nicotine and Its Metabolites, and Their Half-Lives in Urine

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  • MATSUKI Hideaki
    Department of Nursing, School of Health Sciences, Tokai University
  • HASHIMOTO Keiji
    Kyoto Pharmaceutical University
  • ARASHIDANI Keiichi
    Department of Environmental Management, School of Health Sciences, University of Occupational and Environmental Health
  • AKIYAMA Yukio
    Department of Environmental Management, School of Health Sciences, University of Occupational and Environmental Health
  • AMAGAI Takashi
    Institute for Environmental Sciences, University of Shizuoka
  • ISHIZU Yoshiaki
    Department of Socio-Environmental Design, Faculty of Infrastructual Technologies, Hiroshima International University
  • MATSUSHITA Hidetsuru
    Shizuoka Institute of Environment and Hygiene

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Other Title
  • 尿中ニコチンおよびニコチン代謝産物の簡易同時定量法と半減期

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Description

The objectives of this research were to improve and standardize a relatively easy, highly sensitive and highly accurate method of measuring nicotine, cotinine and trans-3'-hydroxycotinine in the urine of non-smokers exposed to environmental tobacco smoke (ETS) and to clarify the reliability of this method. Blinded studies using this analytical method were conducted in two universities. Standard solutions of nicotine, cotinine and trans-3'-hydroxycotinine were prepared at one university, divided in two parts and sent to another two universities for analyses by gas chromatography-mass spectrometry (GC/MS) without revealing the concentrations. It was found that the assay lower limit was at a level that could be used in passive smoking surveys and good results were obtained in crosschecks of samples of unknown concentration between the two universities. Since this method was considered to be useful for analyzing these urinary substances, ETS exposure experiments were performed in three universities using urinary nicotine, cotinine and trans-3'-hydroxycotinine as specific biomarkers of the urine. Non-smokers were exposed to ETS in an exposure room in each university. It was found that the nicotine concentrations in the urine of the subjects exposed to ETS reached a peak at about 2 hours after the end of exposure, which was somewhat later than that in active smokers. Because cotinine and trans-3'-hydroxycotinine in the urine are metabolites of nicotine, it was evident that the quantities were lower and the increasing rates were also less than that of nicotine. When the deceases in nicotine/ creatinine, cotinine/creatinine and trans-3'-hydroxycotinine/creatinine ratios in the urine were calculated using theoretical curves, the half-life times were calculated to be 13.9, 20.0 and 63.0 hours, respectively.

Journal

  • Journal of UOEH

    Journal of UOEH 30 (3), 235-252, 2008

    University of Occupational and Environmental Health, Japan

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