Identification and Antioxidant Activity of Several Pigments from the Residual Green Tea (<I>Camellia sinensis</I>) after Hot Water Extraction
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- HIGASHI-OKAI Kiyoka
- Department of Food and Nutrition, Hiroshima Jhogakuin University
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- YAMAZAKI Masako
- Department of Food and Nutrition, Hiroshima Jhogakuin University
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- NAGAMORI Hiroko
- Department of Food and Nutrition, Hiroshima Jhogakuin University
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- OKAI Yasuji
- Department of Human Life Science, Osaka Kun-Ei Women's College
Bibliographic Information
- Other Title
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- お茶ガラに存在する抗酸化活性について
- Identification and Antioxidant Activity of Several Pigments from the Residual Green Tea(Camellia sinensis) after Hot Water Extraction
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Abstract
Antioxidant activity of green tea extract or tea-derived polyphenols has been extensively studied. However, antioxidant activity in the non-polyphenolic fraction of green tea has been poorly analyzed. In the present study, we analyzed the antioxidant activity of the non-polyphenolic fraction of the residual green tea (Camellia sinensis) after hot water extraction using the aluminum chloride method. The non-polyphenolic fraction of residual green tea caused a significant suppression against hydroperoxide generation from oxidized linoleic acid in a dose-dependent manner. When the concentrate of the non-polyphenolic fraction was applied to a silica gel TLC plate and developed, six color spots were observed, which were considered to be chlorophylls a and b, pheophytins a and b, carotenoids, such as β-carotene and lutein according to their specific colors, Rf values of silica gel TLC and spectrophotometric properties. Among these pigments, pheophytins a and b showed relatively abundant amounts, and the second major group of the pigment was chlorophylls a and b, and carotenoids such as β-carotene and lutein indicated lower concentrations. Although all these pigments exhibited significant antioxidant activities, the ranks of suppressive activity against hydroperoxide generation were chlorophyll a > lutein > pheophytin a > chlorophyll b > β-carotene > pheophytin b. These results suggest that the non-polyphenolic fraction of residual green tea has a potent suppressive activity against hydroperoxide generation from oxidized linoleic acid, which is derived from the antioxidant activities of chlorophylls a and b, pheophytins a and b, β-carotene and lutein. This finding also implys that the combined intake of polyphenols in water-soluble fraction and antioxidative pigments in the non-polyphenolic fraction of green tea will be more efficient to prevent life style-related chronic diseases.
Journal
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- Journal of UOEH
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Journal of UOEH 23 (4), 335-344, 2001
The University of Occupational and Environmental Health, Japan
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Details 詳細情報について
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- CRID
- 1390001205084287488
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- NII Article ID
- 110001260153
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- NII Book ID
- AN0009832X
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- ISSN
- 21872864
- 0387821X
- http://id.crossref.org/issn/0387821X
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- NDL BIB ID
- 6003979
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed