Comparison of Three <I>in vitro</I> Assay Systems Used for Assessing Cytotoxic Effect of Heavy Metals on Cultured Human Keratinocytes

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  • YAMAMOTO Osamu
    Department of Dermatology and Occupational Dermatopathology, School of Medicine, University of Occupational and Environmental Health
  • HAMADA Tetsuo
    Department of Pathology and Molecular Biology, School of Medicine, University of Occupational and Environmental Health
  • TOKUI Noritaka
    Department of Clinical Epidemiology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health
  • SASAGURI Yasuyuki
    Department of Pathology and Molecular Biology, School of Medicine, University of Occupational and Environmental Health

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Other Title
  • 培養成人ケラチノサイトへの重金属の細胞毒性についての3種の<I>in vitro</I>アッセイの比較研究
  • Comparison of Three in vitro Assay Systems Used for Assessing Cytotoxic Effect of Heavy Metals on Cultured Human Keratinocytes

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Description

The cell viability assay using cultured cells is of great advantage to elucidate the biological effect of potentially toxic substances. Recently, a novel assay system, Tetracolor One cell proliferation assay (Seikagaku Co., Tokyo, Japan), has been developed. In this report, we compare the results of the Tetracolor One assay regarding the cytotoxic effect of three heavy metal salts on cultured adult keratinocytes to those of the neutral red dye uptake assay and the MTT eluted stain assay. In this study, these three methods showed almost similar results. Compared to the other two methods, however, the Tetracolor One assay, which requires only one-step procedure before spectrophotometric measurement, is easier to use, and errors in measurement, which may be produced through the multistep procedure, are much less in this assay. Therefore, we believe that the Tetracolor One assay system is useful for assessing the cytotoxic effect of heavy metals on cultured human keratinocytes.

Journal

  • Journal of UOEH

    Journal of UOEH 23 (1), 35-44, 2001

    University of Occupational and Environmental Health, Japan

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