Ca<sup>2+</sup> Imaging in Perfused Adrenal Medullae

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  • WARASHINA Akira
    Department of Cell and Systems Physiology, School of Medicine, University of Occupational and Environmental Health, Japan.
  • INOUE Masumi
    Department of Cell and Systems Physiology, School of Medicine, University of Occupational and Environmental Health, Japan.

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Other Title
  • 潅流副腎髄質におけるCa<sup>2+</sup>イメージング
  • Ca²⁺ Imaging in Perfused Adrenal Medullae

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Abstract

The Ca2+ imaging method was developed to explore changes in excitability in adrenal medullary (AM) cells in a large field in response to synaptic input and chemicals. The adrenal medullae of rats and guinea pigs were retrogradely loaded with Ca2+ indicator through the adrenal vein. Nerve fibers remaining in the adrenal gland were electrically stimulated to induce postsynaptic responses in AM cells, and chemicals were applied to the cells by adding to the perfusate. With this method, γ-aminobutyric acid (GABA) was shown to increase the Ca2+ signal in almost all and 40% AM cells in guinea pigs and rats, respectively.

Journal

  • Journal of UOEH

    Journal of UOEH 34 (2), 163-173, 2012

    The University of Occupational and Environmental Health, Japan

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