10. Protecting Effects of Saccharides on Freeze Damage of Vesicles : A Study by Fluorescence Quenching Method(Papers presented at the 54^<th> Annual Meeting, June 14, 2008, Ishikawa)

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  • NAGASAWA Yutaka
    Division of Frontier Materials Science, Graduate School of Engineering Science, and Center for Quantum Science and Technology under Extreme Conditions, Osaka University
  • YANE Takako
    Division of Frontier Materials Science, Graduate School of Engineering Science, and Center for Quantum Science and Technology under Extreme Conditions, Osaka University
  • ITO Syoji
    Division of Frontier Materials Science, Graduate School of Engineering Science, and Center for Quantum Science and Technology under Extreme Conditions, Osaka University
  • MIYASAKA Hiroshi
    Division of Frontier Materials Science, Graduate School of Engineering Science, and Center for Quantum Science and Technology under Extreme Conditions, Osaka University

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  • 10. ベシクルの凍結破壊に対する糖類の保護効果 : 蛍光消光法による研究(平成20年度第54回低温生物工学会年会)
  • ベシクルの凍結破壊に対する糖類の保護効果--蛍光消光法による研究
  • ベシクル ノ トウケツ ハカイ ニ タイスル トウルイ ノ ホゴ コウカ ケイコウ ショウコウホウ ニ ヨル ケンキュウ

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Abstract

Fluorescence quenching is a simple method to determine the amount of molecules trapped inside phospholipid bilayer vesicles. It utilizes a fluorescent dye, calcein, the fluorescence of which can be quenched by chilate formation with cobalt (II) ions. The amount of calcein trapped inside POPC vesicles before and after freezing in presence and absence of saccharides was compared by this method. Leakage of the interior due to the damage caused by freeze-thaw process was detected. Three sacchrides were examined and sucrose exhibited the strongest protective ability while glucose exhibited the weakest. Trehalose was not very effective when it was added only in the exterior. However, the protective ability became comparable to that of sucrose when it was added to both interior and exterior of the vesicle.

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