書誌事項
- タイトル別名
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- Structure and engineering of CRISPR-Cas9
- CRISPR-Cas9 ノ リッタイ コウゾウ ト キノウ カイヘン
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説明
<p>In the microbial type-II CRISPR-Cas （clustered regularly interspaced short palindromic repeat and CRISPR-associated）adaptive immune system, the RNA-guided DNA endonuclease Cas9 is responsible for the degradation of foreign genetic elements. Using dual RNA guides or a single chimeric RNA guide, Cas9 can recognize and cleave a double-stranded DNA target complementary to the RNA guide. Thus, Cas9 has been harnessed for numerous technologies, such as genome editing, epigenome editing and chromosome imaging. Recent structural studies provided mechanistic insights into the RNA-guided DNA recognition and cleavage by Cas9. The crystal structures revealed that Cas9 adopts a bilobed architecture, and recognizes the guide RNA-target DNA heteroduplex within the central channel between the two lobes in a non-sequence-specific manner, thereby explaining the RNA-guided DNA recognition by Cas9. In addition, the structures revealed that the HNH and RuvC endonuclease domains are located at positions suitable for cleaving the two strands in the unwound DNA target. Furthermore, the structural information facilitated the development of engineered Cas9 variants, such as those with enhanced cleavage fidelity or altered DNA-targeting specificities.</p>
収録刊行物
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- 植物の生長調節
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植物の生長調節 52 (1), 11-14, 2017
一般社団法人 植物化学調節学会
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詳細情報 詳細情報について
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- CRID
- 1390001205086002304
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- NII論文ID
- 130006884417
- 40021236805
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- NII書誌ID
- AA11550064
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- ISSN
- 21896305
- 13465406
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- NDL書誌ID
- 028318459
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
