高速度カメラを用いた植物の細胞内凍結における氷結晶形成挙動

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  • Ice Crystal Formation Behaviors at Intracellular Freezing of Plant using High Speed Camera
  • コウソクド カメラ オ モチイタ ショクブツ ノ サイボウ ナイ トウケツ ニ オケル コオリ ケッショウ ケイセイ キョドウ

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Cryopreservation to preserve biomaterial in a living state over the long term is an essential technique in various fields including medical and food engineering. However, intracellular freezing after extracellular freezing during cryopreservation of fresh vegetables with often used cooling rate causes lethal cell damage due to intracellular ice formation. Therefore, it is important to understand the intracellular freezing mechanism to improve the cryopreservation technique. This study was performed to clarify the characteristic intracellular ice crystal formation behavior in plant tissues using a high speed camera. The results showed that behavior clearly varied with cooling rate. The high speed camera images revealed the quantitative differences in the growth rate and grain diameter of intracellular ice crystals with respect to the cooling rate.

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