Dynamics of endoplasmic reticulum during freezing in living plant cells

DOI IR Web Site 5 References
  • KOBAYASHI Shion
    Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University
  • TANINO Karen
    Department of Plant Sciences, University of Saskatchewan
  • UEMURA Matsuo
    Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University
  • KAWAMURA Yukio
    Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University

Bibliographic Information

Other Title
  • 植物細胞における小胞体凍結動態の観察
  • ショクブツ サイボウ ニ オケル ショウホウタイ トウケツ ドウタイ ノ カンサツ

Search this article

Abstract

Many plants living under subzero temperatures in winter increase freezing tolerance by exposure to non-freezing temperature, which is known as cold acclimation. In cold-acclimated cells, unique cryobehaviors of the plasma membrane and endoplasmic reticulum (ER) have been reported but their physiological meaning or mechanism is largely unknown. Allium fistulosumis a cold-hardy Welshonion which survives winter of-40℃ in Saskatchewan, Canada, andintact cells inthe single epidermal layer, which is easily peeled from leaf sheath, were observed. The cryobehavior of ER in these epidermal cells that were stained with ER-selective fluorescent dye (ER-Tracker) was observed using a confocal fluorescent microscope with cryostage. According to our observations, cold acclimation increased ER volume per cell and extracellular freezing induced ER vesiculation through the breakdown of the ER network. Freeze-induced ER vesicles in cold-acclimated cells were larger and more abundant than those in non-acclimated cells. ER vesiculation may be associated with extracellular calcium because freeze-induced ER vesicles tended to be more abundant in the presence of calcium than in the absence of calcium. Furthermore, ER vesiculation also occurred in Arabidopsis root cells, suggesting a possibility that ER vesiculationis conserved in monocotyledonous and dicotyledonous plants. After thawing, the ER network was recovered only in cold-acclimated cells, suggesting that the dynamics of ER during freeze/thaw cycles are associated with freezing tolerance.

Journal

References(5)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top