16. Vigorous Growth on Plants Regenerated from Cryopreserved Tissue(the 48th Annual Meeting)

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  • 16. 凍結保存後の組織から再生した植物体に認められる旺盛な生長(平成14年度第48回低温生物工学会研究報告)
  • 凍結保存後の組織から再生した植物体に認められる旺盛な成長
  • トウケツ ホゾン ゴ ノ ソシキ カラ サイセイ シタ ショクブツタイ ニ ミトメラレル オウセイ ナ セイチョウ

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Abstract

During this work, We defined that vigorous growth on cryopreserved asparagus (Asparagus officinalis L.) regenerates were induced not by cryoprotectant, but by frozen-thawed treatment with different cryoprotectant 0.6M raffinose. And lateral buds of asparagus nodal segments freezing treated were dyed more deeply and extensively by 0.1% (w/v) 2, 3, 5-Triphenyl tetrazolium chloride (TTC) reduction pigment as time goes by. Histological examination showed that mitochondria were increasing after cryopreservation in the treated cell. Enhansed growth were observed by horseradish with slow freezing method, which was supposed that vigorous growth by cryopreservation might exist generally.

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