修飾1%小川卵黄培地による癩菌培養

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  • Cultivation of <i>Mycobacterium leprae</i> on Modified 1% Ogawa Yolk Media
  • シュウショク 1パーセント オガワ ランオウ バイチ ニヨル ライキン バイヨ

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Follow-up experiments of Ogawa's method for cultivation of Mycobacterium lepraemurium were succeeded by Kozeki and Mon. This steadfast method of isolation of M. lepraemurium might have been established today. Now it is urgently necessary that this method shold be applied to cultivation of Mycobacterium leprae encouraging by the success in case of M. lepraemurium. As it was already thought from a biochemical study of M. lepraemurium that M. lepraemurium might be injured by the excess of oxygen and Prabharan reported that M. leprae have a diphenol oxidase and the diphenol might have an important role in the metabolism of M. leprae, some suitable reducing agents and diphenol compounds must be used in culture of M. leprae.<br>Inhibition testes for some reducing agents and diphenol compounds were done by using M. lepraemurium and 1% Ogawa yolk medium. As seen in Table 1 and Table 2, the suitable concentrations of DOPA, cystein, thioglycolate and adrenalin were respectively 3lγ-62γ, 31γ, 15γ-31γ and 10γ per ml. The other redusing and modified reagents were unsuitable to the growth of M. lepramuriume. Then the leproma materials were cultivated for one year at 30°-35°C. on 1% Ogawa yolk media modifield with 33.3γ/ml DOPA, 33.3γ/ml 1-cystein, 17γ/ml thioglycolate and l0γ/ml 1-adrenalin. Eight materials from 8 leprosy patients in National Leprosarium Airakuen, 3 materials from one patient in National Leprosarium Nanseien, one material sent from Professor Nakamura, one material given from National Leprosarium Seishoen and 3 meterials from 3 new patients in our clinic were cultured. The results of all experiments were negative even cultivating under the 5% CO2, 1% oxygen and nitrogen condition.

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