マウスの皮下における鼠癩菌増殖像の伸展標本による観察

DOI Web Site

書誌事項

タイトル別名
  • Observation of <i>M. leraemurium</i> in Subcutaneous Tissue of Mice by Spread Tissue Preparations
  • マウス ノ ヒカ ニ オケル ネズミライキン ゾウショクゾウ ノ シンテン ヒ

この論文をさがす

抄録

Mice of 6 inbred strains (C3H, CF#1, BALB/C, KK, DDD and C57BL/6) were inoculated subcutaneously in the back with 0.25 ml of a 1:1000 leproma suspension (Hawaiian strain). Spread tissue preparations were made from the inoculation site at about weekly intervals until 10 weeks to observe growth patterns of murine leprosy bacilli in subcutaneous tissues.<br>There were no remarkable differences among these 6 strains during the first 3 weeks after inoculation. In 1 week, an acute inflammatory reaction with polymorphonuclears disappeared and elongation of the bacilli without increase in number was observed in mononuclears. The bacilli showed longer and thinner forms with a length of about 2 to 3 times the initial size. At 3 weeks, enlarged mononuclears, being crowded with the long bacilli, could easily be demonstrable by low magnification.<br>Four weeks after inoculation, however, significant differences were observed in growth patterns of murine leprosy bacilli among these mouse strains. In C3H and CF#1 mice, inflammatory cells were consisted mainly of mononuclears, most of which were heavily loaded with the long bacilli. On the contrary, in the other 4 strains, many lymphocytes and polymorphonuclears were found in the subcutaneous inoculation site, surrounding a smaller number of the bacillus-containing mononuclears. Such differences between mouse strains became more remarkable at 5 weeks, because of more pronounced cellular reactions in these 4 strains.<br>The significant differences between C3H and CF#1 mice were manifested in 10 weeeks. In CF#1 mice lymphocyte infiltration, which surrounded the lesions of mononuclears containing the bacilli, was evident, whereas no host cellular reactions were seen in C3H mice.<br>From the results of these observations, this experimental method can be recommended for early evaluation of development of mouse leprosy, with special reference to the relation of the host cells to the organism, and these mouse strain differences are presumably due to the cell-mediated immunity developed in the hosts.

収録刊行物

詳細情報 詳細情報について

問題の指摘

ページトップへ