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- 中村 昌弘
- 久留米大学医学部微生物学教室細菌学講座
書誌事項
- タイトル別名
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- Multiplication of Mycobacterium lepraemurium in Cell-free Liquid Medium
- ム サイボウ エキタイ バイチ ニ オケル ネズミライキン ノ ゾウキン 13
- 13. Growth Evaluation; Sediment Smear Method (SSM)
- 第13報 増菌判定法,SSM (Sediment Smear Method)の紹介
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抄録
To evaluate the growth rate of M. lepraemurium on the course of cultivation in cellfree liquid medium, a new estimation method for this purpose was established, and named the Sediment Smear Method (SSM). The procedure is as follows: centrifugalize culture tubes at 2, 500 rpm for 30 min, discard carefully the culture fluid by a capillary pipet, resuspended collected sediment in 0.1 ml of a formaline-gelatin solution, smear this suspension by a loop on a slide glass, and stain, then observe the bacterial mass under the low magnification. The results obtained illustrate there is roughly a parallel relationship with the increase of the number of bacilli measured by a bacillary counting method. Therefore, the SSM could be recommended as a simple screening method for course evaluation of the growth of M. lepraemurium, may be as well as M. leprae, in the cellfree cultivation experiments.
収録刊行物
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- 日本らい学会雑誌
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日本らい学会雑誌 46 (3), 98-103, 1977
日本ハンセン病学会
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詳細情報 詳細情報について
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- CRID
- 1390001205094667136
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- NII論文ID
- 130003754732
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- NII書誌ID
- AN00198437
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- ISSN
- 21851360
- 03863980
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- NDL書誌ID
- 1913597
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- PubMed
- 361673
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可