Genome-Wide Screening of Laser Capture Microdissected Gastric Signet-Ring Cell Carcinomas
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- Kurihara Yuji
- Department of Molecular Pathology, Institute of Gerontology, Nippon Medical School Center for Digestive Diseases, Second Hospital, Nippon Medical School
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- Ghazizadeh Mohammad
- Department of Molecular Pathology, Institute of Gerontology, Nippon Medical School
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- Bo Hideki
- Department of Molecular Pathology, Institute of Gerontology, Nippon Medical School Center for Digestive Diseases, Second Hospital, Nippon Medical School
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- Shimizu Hajime
- Department of Molecular Pathology, Institute of Gerontology, Nippon Medical School
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- Kawanami Oichi
- Department of Molecular Pathology, Institute of Gerontology, Nippon Medical School
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- Moriyama Yukichi
- Center for Digestive Diseases, Second Hospital, Nippon Medical School
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- Onda Masahiko
- First Department of Surgery, Nippon Medical School
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説明
Gastric signet-ring cell carcinoma comprises a distinct category of gastric cancers and has been reported to have poor prognosis. In an attempt to define genetic changes involved in the pathogenesis of this lesion in an in vivo state, we isolated signet-ring cell carcinoma cells from freshly fixed smears of tumor tissues of 7 primary gastric signet-ring cell carcinomas by laser capture microdissection and applied comparative genomic hybridization (CGH) to screen for DNA sequence copy number changes. Frequent chromosomal gains were detected on 2q, 5p, 7q, 14q and 20q, each in 6/7 cases, on 9q, 12q, 17q, and 19q, each in 5/7 cases, and on 18p in 4/7 cases. Frequent losses were observed on 6p and 17p, each in 5/7 cases, on 6q, and 21p, each in 4/7 cases, and on 3p, 8p and 8q, each in 3/7 cases. Losses on 6p have rarely been observed in conventional types of gastric carcinomas reported in the literature. These data provide the first evidence for the occurrence of specific genomic aberrations in gastric signet-ring cell carcinomas. Our observation of frequent losses on 6p chromosomal arm may provide novel abnormalities of potential significance in gastric signet-ring cell carcinomas, suggesting the involvement of genes residing in this region in the genesis of the disease.<br>
収録刊行物
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- 日医大誌
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日医大誌 69 (3), 235-242, 2002
日本医科大学医学会
