麻疹不活化ワクチンに関する研究

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書誌事項

タイトル別名
  • Studies on Inactivated Measles Vaccine
  • マシン フカツカ ワクチン ニ カンスル ケンキュウ 1 マシンセイ ワクチン ト フカツカ ワクチン ノ コウゲンセイ ノ ヒカク
  • 1. A Comparative Study on Antigenicity of Live and Inactivated Measles Vaccines
  • 第1報 麻疹生ワクチンと不活化ワクチンの抗原性の比較

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1) No differences were revealed in hemagglutination inhibiting (HI) and neutralizing activities between live measles vaccine and the one inactivated with sodium ethylmercurithiosalicylate, when they were titered above a certain threshold. 2) Upon comparison of antigenic extinction limits (AEL) of live and inactive vaccines by their inoculation into guinea pigs, there were no differences at 1 and 2 months following inoculation. Thereafter, however, the AEL of the live vaccine were much higher than those of the inactivated. The neutralizing antibody responses in guinea pigs inoculated with the highest dilution of the live vaccine were then the same as those inoculated with the undiluted vaccine. In the groups inoculated with inactivated vaccine, such a phenomenon was not observed. The degrees and courses of the neutralizing and CF antibody responses of the animals inoculated with both “undiluted ”vaccines were similar. 3) The titration of antibodies was carried out on HI and neutralizing activities of sera collected from rabbits at 4 and 12 weeks after three doses hyperimmunization of live and/or inactivated vaccine. The antibody responses in Peak 1 of gel-filtrations on Sephadex G-200 of the sera from the animals inoculated with live and/or inactivated vaccine decreased with the lapse of time, while those in Peak 2 gradually increased to a much higher level -than those of Peak 1. The serum of the rabbit inoculated with inactivated vaccine was titered much higher in both HI and neutralizing antibodies than that of the rabbit inoculated with live vaccine. 4) Antibody titrations were done also on HI and neutralizing activities of sera collected from guinea pigs at seven, 14 and 21 days after one dose inoculations of live and/or inactivated vaccine. The antibody responses in Peak 1 of gel-filtration on Sephadex G-200 of the sera were at the highest level in 7- or 14-days sera and definitely decreased in 21-days sera, while those in Peak 2 were at the highest level in 14 days sera and slightly decreased in 21-days sera. 5) The early sera following vaccine inoculation or fractions of the sera in Peak 1 of gel-filtration on Sephadex G-200 were destroyed easily by the sulfhydryl reagent 2-mercaptoethylamine, while the 21-days sera or serum fractions in Peak 2 of the gelfiltration were stable to the reagent. These facts suggest that Peaks 1 and 2 of gel-filtration on Sephadex G-200 of sera contain mainly 19S and 7S antibodies, respectively.

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