キダチアロエ, アロエベラおよびアロエアフリカーナのマイトジェン活性物質の検索

DOI 日本農学文献記事索引 Web Site 参考文献6件

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タイトル別名
  • Study on mitogenic activity components of <I>Aloe arborescens</I> Miller, <I>Aloe barbadensis</I> Miller and <I>Aloe africana</I> Miller
  • キダチ アロエ アロエ ベラ オヨビ アロエ アフリカーナ ノ マイトジェン カッセイ ブッシツ ノ ケンサク

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Previously, we have reported on the mitogenic activity of Aloe arborescens Miller, Aloe barbadensis Miller, and Aloe africana Miller. Six fractions, the non-dialyzable fraction of hot water extracts (KS-F2) from the skin of Aloe arborescens Miller, the dialyzable fraction of hot water extracts (BF-F1) from the flesh of Aloe barbadensis Miller, the dialyzable fraction of hot water extracts (AF-F1), the non-dialyzable fraction of 0.1 M NaOH extracts (AF-F3), From the flesh of Aloe africana Miller, and the non-dialyzable fraction of hot water extracts (AS-F2), and the non- dialyzable fraction of 0.1M NaOH extracts (AS-F3) from the skin of Aloe africana Miller, showed mitogenic activity towards cells from mice (_??_), C3H/HeN and C3H/HeJ. In this paper, we report on some properties of polysaccharide fractions from which mitogenic activity was shown. A polysaccharide obtained from KS-F2 and AS-F2 which showed strong mitogenic activity with de protein was fractionated and purified by ion-exchange chromatography by DEAE-Toyopearl 650 M. Each of two fractions from KS-F2 mainly contained neutral sugar, and uronic acid. All of three fractions from AS-F2 contained neutral sugar, and one of them contained uronic acid. These results of mitogen activity assays for the major fractions suggested that the activity components of AS-F2 were located in the fraction containing uronic acid, and those of AS-F2 were located in the fraction that had greater amounts of polysaccharides, such as neutral sugar and uronic acid, than the other fractions. A polysaccharide obtained from six fractions was examined regarding the components and molar ratio of sugar by gaschromatography. Its fraction were composed of large amounts of arabinose and galactose.

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