Structural studies of mitogenic activity components of <I>Aloe arborescens</I> Miller, <I>Aloe barbadensis</I> Miller and <I>Aloe africana</I> Miller

DOI JASI Web Site 12 References

Bibliographic Information

Other Title
  • キダチアロエ, アロエベラおよびアロエアフリカーナのマイトジェン活性物質の化学構造解析
  • キダチアロエ アロエベラ オヨビ アロエアフリカーナ ノ マイトジェン カッセイ ブッシツ ノ カガク コウゾウ ブンセキ

Search this article

Abstract

Previously, we reported that KS-F2 (non-dialyzable fraction of hot water extracts from skin of Aloe arborescensMiller) and AS-F2 (non-dialyzable fraction of hot water extracts from skin of Aloe africanaMiller) with de-protein showed a high mitogenic activity towards cells of mice (_??_) (C3HeN/C3HeJ). The water-soluble polysaccharides obtained were fractionated and purified by ion-exchange chromatography on a DEAE-Toyopearl 650M column. The chemical structres of K-1, the mitogenic activity component of KS-F2 and A-1, those of AS-F2, were analyzed. Arabinose and galactose were contained in both K-1 and A-1. The molar ratio of arabinose to galactose was 1 : 1 in K-1, and 1 : 2 in A-1 before reduction and 1 : 3 in K-1, and 1 : 3 in A-1 after reduction. The ratio of galactose increased both K-1 and A-1. Consequently, uronic acid seemed to be galacturonic acid. The ratio of neutral sugar to uronic acid contents in K-1 and A-1 was about 1 : 1 and 5.6 : 1, respectively. K-1 and A-1 were found as gel-filtration fractions with a high molecular weight of approximately 1, 700, 000 and 1, 750, 000, respectively. The main components in K-1 were found to be arabinose and galactose based on their [α] D value of +127 (H2O) and a peak of 890 in IR ν max cm-1. A-1 also showed arabinose and galactose based on their [α] D value of-33 (H2O) and a peak of 830 in IR ν max cm-1. Therefore, K-1 seemed to be a β-glucosidic bond, and A-i was close to a β-glucosidic bond. After the controlled hydrolysis of K-1 and A-1, an investigation of the bond form was performed using thin layered chromatography. It was suggested that arabinose and uronic acid were located on the outside and that arabinose and galactose constituted the core of the K-1 and A-1 structures. Using GC/MS, the methylation analysis gave 1, 4-di-O-acetyl-2, 3, 5-tri-O-methyl arabitol, 1, 5-di-O-acetyl-2, 3, 4, 6-tetra-O-methyl galactitol, 1, 3, 5-tri-O-acetyl-2, 4-di-O-methyl arabitol, 1, 3, 5-tri-O-acetyl-2, 4, 6-tri-O-methyl galactitol, 1, 5, 6-tri-O-acetyl-2, 3, 4-tri-O-methyl galactitol and 1, 2, 5, 6-tetra-O-acetyl-3, 4-di-O-methyl galactitol in the molar ratios of 4.3 : 3.4:1.0:3.1:1.9:6.0 from the K-1 core, and 2.4:2.5:1.0:3.8:1.1:2.8 from the A-1 core, respectively.

Journal

  • Food Preservation Science

    Food Preservation Science 26 (3), 145-152, 2000

    Japan Association of Food Preservation Scientists

References(12)*help

See more

Details 詳細情報について

Report a problem

Back to top