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- 久保田 しのぶ
- Research Institute of Nihon Shokuhin Kako Co.
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- 高田 正保
- Research Institute of Nihon Shokuhin Kako Co.
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- 小川 浩一
- Research Institute of Nihon Shokuhin Kako Co.
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- 中村 信之
- Research Institute of Nihon Shokuhin Kako Co.
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- 碓氷 泰市
- Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University
書誌事項
- タイトル別名
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- Novel Enzymatic Synthesis of β-D-Galactosyl- and 6-O-Benzyl-Derivatives of p-Nitrophenyl α-Maltopentaoside
- Novel Enzymatic Synthesis of ベータ D Gala
この論文をさがす
抄録
The hydrolysis of various β-1, 4-galactosyl-maltooligosaccharides (β-1, 4-Gal-Gn) by the action of an exo-maltotetraohydrolase (EC 3.2.1.60, G4-amylase) from Pseudomonas stutzeri was examined to obtain information on action mode and kinetic parameters. The enzyme split 2 points of the. α-1, 4-glucosidic linkage in β-1, 4-galactosyl-maltotetraose (β-1, 4-Gal-G4) and 3 points in β-1, 4-Gal-G5, -Gal-G6 and -Gal-G7 to form various molar ratios of β-1, 4-Gal-G2, -Gal-G3 and -Gal-G4, respectively, and larger ko/Km values were observed with larger β-1, 4-Gal-Gn as substrates. The G4-amylase catalyzed the formation of p-nitrophenyl β-1, 4-D-galactosyl-α-maltopentaoside (β-1, 4-Gal-G5P) by its transfer action with a yield of 3.3, 11.3 and 6.8% by weight, and coincided well with the hydrolytic pattern of β-1, 4-Gal-Gn (n=5-7), from the mixture of p-nitrophenyl α-D-glucopyranoside (GP) and β-1, 4-Gal-G5, -Gal-G6 or -Gal-G7, respectively. The G4-amylase synthesized β-1, 4-Gal-G5P with a yield of 6.1% by weight of the mixture of GP with crude Gal-Gn containing primarily Gal-G6 and Gal-G7, of which about 10% was the respective β-1, 6 isomer. By an analogous method, the preparation of p-nitropheny16-0-benzyl-α-maltopentaoside (BG5P) was also examined with the mixture of GP and methyl 6-O-benzyl-α-maltooligosaccharides (BGn-Me) containing primarily BG6-Me and BG7-Me. In contrast to the action on Gal-Gn, the enzyme split 1 point of the α-1, 4-glucosidic linkage in BGn-Me, and the BG4 formed was readily transferred to the 4-position of GP by the α-1, 4-glucosidic linkage with a yield of 10.2% (w/w) . By these methods, the complicated procedures to obtain pure p-nitrophenyl α-malto-pentaoside (G5P) were eliminated from the conventional production methods of Gal-G5P and BG5P.
収録刊行物
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- 応用糖質科学
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応用糖質科学 44 (3), 313-321, 1997
日本応用糖質科学会
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詳細情報 詳細情報について
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- CRID
- 1390001205170205568
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- NII論文ID
- 10008257205
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- NII書誌ID
- AN10453916
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- COI
- 1:CAS:528:DyaK2sXmsVWjsL4%3D
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- ISSN
- 18844898
- 13403494
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- NDL書誌ID
- 4304125
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可