Arthrobacter globifoYrnis T6 Isomaltodextranase Transfers Isomaltosyl Residue from Dextran

DOI
  • KIM Yeon-Kye
    Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • SAKANO Yoshiyuki
    Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology

Bibliographic Information

Other Title
  • Arthrobacter globiformis T6 イソマルトデキストラナーゼはデキストランより遊離されるインマルトシル基を受容体のC-4位へも転移する

Abstract

Transfer action of isomaltodextranase [EC 3.2.1.94] from Arthrobacter globiformis T6 was studied using glucose and maltose as acceptors. This enzyme transferred an α-isomaltosyl residue from dextran to the C-4 and C-6 positions at the non-reducing end of acceptors . Thus, the enzyme transfer red isomaltose to glucose and maltose to produce panose and isomaltotriose, and 42-α-isomaltosyl maltose (IMM) and 4-α-isomaltotriosyl D-glucose (IMTG), respectively. The transisomaltosyl activity at the C-4 position was 10-13% of that at the C-6 position in HPLC analysis, and in Fuji Film BAS2000 image analyzer analysis using D- [U-14C] glucose as an acceptor.

Journal

Details 詳細情報について

  • CRID
    1390001205172019200
  • NII Article ID
    130004091090
  • DOI
    10.11541/jag1994.43.35
  • ISSN
    18844898
    13403494
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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