Arthrobacter globifoYrnis T6 Isomaltodextranase Transfers Isomaltosyl Residue from Dextran
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- KIM Yeon-Kye
- Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology
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- SAKANO Yoshiyuki
- Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology
Bibliographic Information
- Other Title
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- Arthrobacter globiformis T6 イソマルトデキストラナーゼはデキストランより遊離されるインマルトシル基を受容体のC-4位へも転移する
Abstract
Transfer action of isomaltodextranase [EC 3.2.1.94] from Arthrobacter globiformis T6 was studied using glucose and maltose as acceptors. This enzyme transferred an α-isomaltosyl residue from dextran to the C-4 and C-6 positions at the non-reducing end of acceptors . Thus, the enzyme transfer red isomaltose to glucose and maltose to produce panose and isomaltotriose, and 42-α-isomaltosyl maltose (IMM) and 4-α-isomaltotriosyl D-glucose (IMTG), respectively. The transisomaltosyl activity at the C-4 position was 10-13% of that at the C-6 position in HPLC analysis, and in Fuji Film BAS2000 image analyzer analysis using D- [U-14C] glucose as an acceptor.
Journal
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 43 (1), 35-41, 1996
The Japanese Society of Applied Glycoscience
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Details 詳細情報について
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- CRID
- 1390001205172019200
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- NII Article ID
- 130004091090
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- ISSN
- 18844898
- 13403494
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed