Bacillus czycuansT-3040株のサイクロイソマルトオリゴ糖グルカノトランスフェラーゼ遺伝子のクローニングと大腸菌での発現
書誌事項
- タイトル別名
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- Cloning and Sequence Analysis of the Cycloisomaltooligosaccharide Glucanotransferase Gene from Bacillus ciyculans T-3040 and Expression in Escherichia coli Cells
抄録
The gene for cycloisomaltooligosaccharide glucanotransferase from Bacillus circulars T-3040 was cloned and expressed in the recombinant plasmid pCI429 in Escherichia coli. The enzyme gene consisted of a unique open reading frame of 2916 bp. Comparison of the DNA sequence data with the N-terminal, C-terminal and 12 internal amino acid sequences of the purified enzyme secreted from B. circulars T-3040 suggested the enzyme was translated from mRNA as a secretory precursor with a signal peptide of 38 amino acid residues. The deduced amino acid sequence of the mature enzyme contained 934 residues, resulting in a polypeptide with a molecular mass of 103, 103 Da. It had no common consensus region which is characteristic of the α-amylase family. The cycloisomaltooligosaccharide glucanotransferase activity, cyclization activity in transformant was about 3.0 mU/ml which was the same as that from B. circulars T-3040.
収録刊行物
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- 応用糖質科学
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応用糖質科学 42 (4), 415-419, 1995
日本応用糖質科学会
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詳細情報 詳細情報について
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- CRID
- 1390001205172022272
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- NII論文ID
- 130004257471
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- COI
- 1:CAS:528:DyaK28XptFKqsg%3D%3D
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- ISSN
- 18844898
- 13403494
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可