山羊の人工受胎に関する研究とくに子山羊の過剰排卵誘起と採取した受精卵の移植試験について

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  • On the induction of superovulation and the development of fertilized ova following to transfer in kids.

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Thirty-one kids of Saanen and Japanese native breed, ranging in age 2 to 15 weeks, were treated with gonadotrophins, PMS and HCG, to induce superovulation. They were inseminated artificially in the uterus with semen from fertile hegoats every 24 hr during oestrus following superovulatory treatments. Ova recovery was performed in vivo or in vitro 3 to 5 days after the HCG injection. Sixteen fertilized ova, which were collected from kids, were transferred surgically to the uterine horn of seven recipients which were adult Saanen goats and synchronized within ± 2 days in oestrus with the donors. The results obtained were as follows.<BR>1) A total of 619 ovulations occurred in twenty-nine of thirty-one treated kids which were 5 to 15 weeks in age. It will be noted that there was a marked increase in number of ovulation points in the ovaries of treated kids which were more than 7 weeks in age.<BR>2) A total of 256 ova was recovered from twenty-four kids except five cases. Only 53 ova from nine kids have been fertilized.<BR>3) Three of seven recipients became pregnant following the ova transfer, and produced five kids, a single or twins per a pregnant recipient, normally.

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