Pharmacological Evidence for Involvement of Phospholipase D, Protein Kinase C, and Sodium-Calcium Exchanger in .ALPHA.-Adrenoceptor-Mediated Negative Inotropy in Adult Mouse Ventricle
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- Nishimaru Kazuhide
- Department of Pharmacology, Toho University School of Pharmaceutical Sciences
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- Tanaka Yoshio
- Department of Pharmacology, Toho University School of Pharmaceutical Sciences
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- Tanaka Hikaru
- Department of Pharmacology, Toho University School of Pharmaceutical Sciences
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- Shigenobu Koki
- Department of Pharmacology, Toho University School of Pharmaceutical Sciences
Bibliographic Information
- Other Title
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- Pharmacological Evidence for Involvement of Phospholipase D, Protein Kinase C, and Sodium-Calcium Exchanger in α-Adrenoceptor-Mediated Negative Inotropy in Adult Mouse Ventricle
- Pharmacological Evidence for Involvement of Phospholipase D Protein Kinase C and Sodium Calcium Exchanger in アルファ Adrenoceptor Mediated Negative Inotropy in Adult Mouse Ventricle
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Abstract
The intracellular signalling pathway for α-adrenoceptor-mediated negative inotropy was studied pharmacologically in isolated adult mouse ventricle. The negative inotropy was inhibited by GF-109203X, a nonselective protein kinase C inhibitor. Phorbol 12-myristate 13-acetate also produced sustained negative inotropy, which was inhibited by KB-R7943, a Na+/Ca2+ exchanger inhibitor. The α-adrenoceptor-mediated negative inotropy was augmented by RHC-80267, a diacylglycerol lipase inhibitor, but was inhibited either by C2-ceramide, a phospholipase D inhibitor, and high concentration of propranolol (50 μM), which inhibits phosphatidate phosphohydrolase. The inotropy was not affected by U-73122, a phospholipase C inhibitor. Lavendustin-A, a tyrosine kinase inhibitor, also inhibited the negative inotropy. These findings suggest that α-adrenoceptor-mediated negative inotropy in adult mouse ventricle is mediated by activation of tyrosine kinase, the phospholipase D-phosphatidate phosphohydrolase pathway, and protein kinase C.<br>
Journal
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- Journal of Pharmacological Sciences
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Journal of Pharmacological Sciences 92 (3), 196-202, 2003
The Japanese Pharmacological Society
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Details 詳細情報について
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- CRID
- 1390001205175573120
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- NII Article ID
- 10011209471
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- NII Book ID
- AA11806667
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- COI
- 1:CAS:528:DC%2BD3sXlvFyqtrw%3D
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- ISSN
- 13478648
- 13478613
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- NDL BIB ID
- 6614235
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- PubMed
- 12890884
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed