Bee Venom Induces Apoptosis and Inhibits Expression of Cyclooxygenase-2 mRNA in Human Lung Cancer Cell Line NCI-H1299

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  • Jang Mi-Hyeon
    Department of Physiology, College of Medicine, Kyung Hee University
  • Shin Min-Chul
    Department of Physiology, College of Medicine, Kyung Hee University
  • Lim Sabina
    Research Group of Pain and Neuroscience in Vision 2000 Project, East-West Medical Research Institute, Kyung Hee Universiy
  • Han Seung-Moo
    Research Group of Pain and Neuroscience in Vision 2000 Project, East-West Medical Research Institute, Kyung Hee Universiy
  • Park Hi-Joon
    Research Group of Pain and Neuroscience in Vision 2000 Project, East-West Medical Research Institute, Kyung Hee Universiy
  • Shin Insop
    Research Group of Pain and Neuroscience in Vision 2000 Project, East-West Medical Research Institute, Kyung Hee Universiy
  • Lee Ji-Suk
    Research Group of Pain and Neuroscience in Vision 2000 Project, East-West Medical Research Institute, Kyung Hee Universiy
  • Kim Kyoung-Ah
    Research Group of Pain and Neuroscience in Vision 2000 Project, East-West Medical Research Institute, Kyung Hee Universiy
  • Kim Ee-Hwa
    Department of Meridianology, College of Oriental Medicine, Semyung University
  • Kim Chang-Ju
    Department of Physiology, College of Medicine, Kyung Hee University Research Group of Pain and Neuroscience in Vision 2000 Project, East-West Medical Research Institute, Kyung Hee Universiy

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To investigate whether bee venom (BV) induces apoptosis, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, terminal deoxynucleotidyl transferase- mediated dUTP nick end-labeling assay, 4,6-diamidino-2-phenylindole staining, flow cytometric analysis, and DNA fragmentation assay were performed on NCI-H1299 lung cancer cells treated with BV. Through morphological and biochemical analyses, it was demonstrated that NCI-H1299 cells treated with BV exhibit several features of apoptosis. In addition, reverse transcription-polymerase chain reaction and prostaglandin E2 (PGE2) immunoassay were performed to verify whether BV possesses an inhibitory effect on the expression of cyclooxygenase (COX) and PGE2 synthesis. Expression of COX-2 mRNA and synthesis of PGE2 were inhibited by BV. These results suggest the possibility that BV may exert an anti-tumor effect on human lung cancer.<br>

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