Ephedorae herba Decreases Lipopolysaccharide-Induced Cyclooxgenase-2 Protein Expression and NF-.KAPPA.B-Dependent Transcription in C6 Rat Glioma Cells

  • Aoki Kohichi
    Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University
  • Yamakuni Tohru
    Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University
  • Yoshida Masaaki
    Research Laboratory, Kotaro Pharmaceutical Co., Ltd.
  • Ohizumi Yasushi
    Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University

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  • Ephedorae herba Decreases Lipopolysaccharide-Induced Cyclooxgenase-2 Protein Expression and NF-κB-Dependent Transcription in C6 Rat Glioma Cells
  • Ephedorae herba Decreases Lipopolysaccharide Induced Cyclooxgenase 2 Protein Expression and NF カッパ B Dependent Transcription in C6 Rat Glioma Cells
  • Ephedorae herba decreases lipoploysaccharide-induced cyclooxgenase-2 protein expression and NF-κB-dependent transcription in C6 rat glioma cells

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Abstract

We studied the effects of Mao (Ephedrae herba) on lipopolysaccharide (LPS)-induced expression of inducible cyclooxygenase (COX-2) in C6 rat glioma cells. Western blot analysis showed that Mao inhibited LPS-induced expression of COX-2 protein, but not that of constitutive cyclooxygenase. Reporter gene assays showed that Mao reduced LPS-inducible NF-κB-dependent transcription that plays a crucial role in induction of COX-2 gene expression. This crude drug decreased LPS-induced IκBα degradation in a concentration-dependent manner without affecting LPS-induced IκB phosphorylation. These results suggest that Mao prevents LPS-induced NF-κB-dependent transcription by inhibiting IκB degradation and suppresses an increase in COX-2 protein expression in C6 cells.<br>

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