Localization of Ca〔2+〕-Activated K〔+〕 Channel, SK3, in Fibroblast-Like Cells Forming Gap Junctions With Smooth Muscle Cells in the Mouse Small Intestine
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- Fujita Akikazu
- Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, Osaka Prefecture University
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- Takeuchi Tadayoshi
- Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, Osaka Prefecture University
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- Jun Hanai
- Department of Pathology and Research, Sakai Municipal Hospital
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- Hata Fumiaki
- Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, Osaka Prefecture University
書誌事項
- タイトル別名
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- Localization of Ca2+-Activated K+ Channel, SK3, in Fibroblast-Like Cells Forming Gap Junctions With Smooth Muscle Cells in the Mouse Small Intestine
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説明
In the present study, we examined the expression and the localization of apamin-sensitive small conductance Ca2+-activated K+ channels (SK channels) in the mouse intestine. SK3-immunoreactivity (IR) was detected in both ileum and colon. Double staining experiments showed that SK3-IR was colocalized with prolyl 4-hydroxylase (PH(α))-IR, but not with c-Kit-IR which are markers of fibroblast cells and the interstitial cells of Cajal (ICC), respectively. Although SK3-IR was colocalized with vimentin-IR, which is another marker of ICC, the reactivity of SK3-immunopositive cells was weaker than that of ICC. The SK3-immunopositive cells were similarly present in the intestine of c-Kit mutant mice (W/WV), in which ICC were absent, and its wild-type mice. The immuno-electron microscopic analysis indicated that SK3 was localized in the cells that had some similar morphological features to ICC, but obviously different from ICC. The SK3-immunopositive cells had gap junctions with the smooth muscle cells. The gap junctions were smaller than those between ICC and smooth muscle cells. These results indicate expression of SK3 in fibroblast-like cells, but not in ICC, and suggest participation of the cells in the intestinal motility.<br>
収録刊行物
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- Journal of Pharmacological Sciences
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Journal of Pharmacological Sciences 92 (1), 35-42, 2003
公益社団法人 日本薬理学会
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詳細情報 詳細情報について
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- CRID
- 1390001205177072512
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- NII論文ID
- 130000073678
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- NII書誌ID
- AA11806667
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- COI
- 1:CAS:528:DC%2BD3sXkt1Sgt7k%3D
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- ISSN
- 13478648
- 13478613
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- NDL書誌ID
- 6536720
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- PubMed
- 12832853
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- PubMed
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