Direct Assessments of the Antioxidant Effects of the Novel Collagen Peptide on Reactive Oxygen Species Using Electron Spin Resonance Spectroscopy

DOI Web Site 6 Citations 55 References
  • Kobayashi Kyo
    Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Japan
  • Maehata Yojiro
    Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Japan
  • Kawamura Yosuke
    Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Japan
  • Kusubata Masashi
    Nippi Research Institute of Biomatrix, Japan
  • Hattori Shunji
    Nippi Research Institute of Biomatrix, Japan
  • Tanaka Keisuke
    Nippi Research Institute of Biomatrix, Japan
  • Miyamoto Chihiro
    Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Japan
  • Yoshino Fumihiko
    Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Japan
  • Yoshida Ayaka
    Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Japan
  • Wada-Takahashi Satoko
    Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Japan
  • Komatsu Tomoko
    Division of Dentistry for Special Patients, Department of Clinical Care Medicine, Kanagawa Dental College, Japan
  • Takahashi Shun-Suke
    Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Japan
  • Lee Masaichi-Chang-Il
    Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Japan

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Abstract

In the present study, we evaluated the antioxidant effects of a pepsin-treated novel collagen peptide (P-NCP) on reactive oxygen species (ROS) such as hydroxyl radical (HO•), superoxide anion radical (O2•–), and singlet oxygen (1O2), and the effects on cell viability after ultraviolet ray (UV) irradiation of human fibroblasts. We confirmed, using electron spin resonance, that P-NCP directly inhibited HO• and 1O2. Furthermore, addition of P-NCP to fibroblasts inhibited cell death induced by UVA (400 – 315 nm) irradiation in a dose-dependent manner. In addition, the antioxidant effect on 1O2 was observed in the peptide fractions rich in Gly, Pro, Hyp, Glu, Ala, and Arg. We found that Gly, Hyp, Glu, and Ala directly scavenged 1O2. These results indicated that a peptide sequence including Gly, Hyp, Glu, and Ala could play a key role in the antioxidant effects of P-NCP on 1O2. It was suggested that P-NCP can inhibit photo-aging related to ROS owing to its antioxidant effects.

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