The Release of Substance P From Cultured Dorsal Root Ganglion Neurons Requires the Non-neuronal Cells Around These Neurons
-
- Tang He-Bin
- Department of Pharmacology, Graduate School of Biomedical Sciences, Hiroshima University, Japan
-
- Li Yu-Sang
- Department of Anatomical Pathology, Hiroshima University Hospital, Japan
-
- Nakata Yoshihiro
- Department of Pharmacology, Graduate School of Biomedical Sciences, Hiroshima University, Japan
Search this article
Abstract
Substance P is known to be released from dorsal root ganglion (DRG) neurons, but the possible involvement of non-neuronal cells in the process of substance P release is not known. During the culture period, the number of surviving DRG neurons, the total substance P content from the culture medium and DRG cells, and the total protein of DRG cells were monitored. Both the number of surviving neurons and the total substance P content decreased in a time-dependent manner, whereas the total protein synthesis was increased. The localization of substance P in small-to-medium–sized neurons was further confirmed by immunocytochemistry. Finally, the substance P release levels from the neuronal and non-neuronal enrichments were investigated by radioimmunoassay. Both partially purified DRG neurons and non-neuronal cells exhibited a weaker substance P release response to capsaicin or KCl, relative to unpurified DRG cells. The total substance P content from the partially purified DRG neurons was almost the same as that from the unpurified DRG cells, but much more than that from the partially purified DRG non-neuronal cells. These findings suggest that substance P is released from DRG neurons, and this process should require the coexistence of neurons and non-neuronal cells.<br>
Journal
-
- Journal of Pharmacological Sciences
-
Journal of Pharmacological Sciences 105 (3), 264-271, 2007
The Japanese Pharmacological Society
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390001205179901056
-
- NII Article ID
- 10024316705
-
- NII Book ID
- AA11806667
-
- ISSN
- 13478648
- 13478613
-
- NDL BIB ID
- 9266591
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- Abstract License Flag
- Disallowed