Tarantula Toxin ProTx-I Differentiates Between Human T-type Voltage-Gated Ca2+ Channels Cav3.1 and Cav3.2
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- Ohkubo Tsuyako
- Department of Physiological Science and Molecular Biology, Fukuoka Dental College, Japan
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- Yamazaki Jun
- Department of Physiological Science and Molecular Biology, Fukuoka Dental College, Japan
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- Kitamura Kenji
- Department of Physiological Science and Molecular Biology, Fukuoka Dental College, Japan
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Abstract
ProTx-I peptide, a venom toxin of the tarantula Thrixopelma pruriens, has been reported to interact with voltage-gated ion channels. ProTx-I reduced Ba2+ currents through recombinant human T-type voltage-gated Ca2+ channels, Cav3.1 (hCav3.1), with roughly 160-fold more potency than through hCav3.2 channels. Chimeric channel proteins (hCav3.1/S3S4 and hCav3.2/S3S4) were produced by exchanging fourteen amino acids in the hCav3.1 domain IV S3-S4 linker region and the corresponding region of hCav3.2 between each other. The ProTx-I sensitivity was markedly reduced in the hCav3.1/S3S4 chimera as compared to the original hCav3.1 channel, while the hCav3.2/S3S4 chimera exhibited greater ProTx-I sensitivity than the original hCav3.2 channel. These results suggest that the domain IV S3-S4 linker in the hCav3.1 channel may contain residues involved in the interaction of ProTx-I with T-type Ca2+ channels.
Journal
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- Journal of Pharmacological Sciences
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Journal of Pharmacological Sciences 112 (4), 452-458, 2010
The Japanese Pharmacological Society
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Details 詳細情報について
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- CRID
- 1390001205180424064
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- NII Article ID
- 10027909166
- 130000253386
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- NII Book ID
- AA11806667
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- ISSN
- 13478648
- 13478613
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- NDL BIB ID
- 10651125
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed