<I>Development of regenerated cardiomyocyte for the cardiovascular tissue engineering</I>
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- Fukuda Keiichi
- Institute for Advanced Cardiac Therapeutics, Keio University School of Medicine
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- Hakuno Daihiko
- Cardiopulmonary Division, Department of Internal Medicine, Keio University School of Medicine
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- Konishi Fusako
- Cardiopulmonary Division, Department of Internal Medicine, Keio University School of Medicine
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- Makino Shinji
- Cardiopulmonary Division, Department of Internal Medicine, Keio University School of Medicine
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- Tomita Yuichi
- Cardiopulmonary Division, Department of Internal Medicine, Keio University School of Medicine
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- Ogawa Satoshi
- Cardiopulmonary Division, Department of Internal Medicine, Keio University School of Medicine
Bibliographic Information
- Other Title
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- 心血管Tissue engineeringを目指した再生心筋細胞の開発
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Abstract
We have isolated a cardiomyogenic cell line (CMG cell) from murine bone marrow stromal cells. Stromal cells were immortalized, treated with 5-azacytidine, and spontaneous beating cells were repeatedly screened for. The cells showed a f ibroblast-like morphology, but the morphology changed after 5-azacytidine treatment in approximately 30% of the cells : they connected with adjoining cells after 1 week, formed myotube-like structures and began spontaneous beating after 2 weeks, and beat synchronously after 3 weeks. They expressed ANP and BNP. Electron microscopy revealed a cardiomyocyte-like ultrastructure including typical sarcomeres and atrial granules. These cells had several types of action potentials : sinus node like and ventricular cell-like action potentials. All cells had a long action potential duration or plateau, a relatively shallow resting membrane potential, and a pacemaker-like late diastolic slow depolarization. Analysis of the isoform of contractile protein genes, such as myosin heavy chain, myosin light chain and α-actin, indicated that their muscle phenotype was similar to fetal ventricular cardiomyocytes. These cells expressed Nkx 2.5/Csx, GATA 4, TEF-1 and MEF-2 C mRNA before 5-azacytidine treatment, and expressed MEF-2 A and MEF-2 D after treatment. This new cell line provides a powerful model for the study of cardiomyocyte differentiation.
Journal
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- Ensho Saisei
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Ensho Saisei 21 (6), 639-644, 2001
The Japanese Society of Inflammation and Regeneration
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Keywords
Details 詳細情報について
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- CRID
- 1390001205181516032
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- NII Article ID
- 130003855388
- 10010036635
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- NII Book ID
- AA11508953
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- ISSN
- 18805795
- 13468022
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed